七种风铃草属植物亲缘关系的ISSR分析

为了解四川风铃草属植物的资源丰富度和遗传进化情况,该研究利用ISSR分子标记,探究了四川7种风铃草属植物的亲缘关系,为风铃草属植物的分子标记辅助鉴定、资源保护、花卉品种的开发与育种提供理论基础。结果表明:40条ISSR引物中有28条引物能够扩增出清晰的条带,扩增总条带数为164,其中有98.8%的扩增条带具有多态性,供试的7种风铃草属植物遗传相似性系数在0.421～0.945之间,其中钻裂风铃草与藏滇风铃草的遗传相似度最高为0.945,说明它们之间的亲缘关系很近。此外,ISSR分子标记聚类结果表明,7种风铃草属植物可以明显聚为4大类:西南风铃草、灰毛风铃草、灰岩风铃草为一类;紫斑风铃草与流石风铃草各成一类,这两种在形态上与其它各种风铃草差异较大;最后是钻裂风铃草与藏滇风铃草为一类。

ISSR analysis on genetic relationships among seven species in Campanula

Genetic relationships among seven species in Campanula L. were investigated by inter-simple sequence repeat molecular markers(ISSR). The results showed that 28 of 40 primers can be amplified to clear bands. All the 28 ISSR primers amplified 164 bands, and the proportion of polymorphic fragment was 98.8%. The value of genetic similarity(GS)indexes of seven species based on the ISSR data varied from 0.421 to 0.945, and the highest genetic similarity was 0.945 between C. aristata and C. modesta, which indicated that they had closely relative relationship. Cluster analysis showed that all the species could be divided into four groups: C. colorata, C. cana and C. calcicola in the first group, C. calcicola in the second group, C. crenulata in the third group, and C. aristata and C. modesta in the last group. This study provides reference for the molecular marker assisted identification, resource conservation, flower variety development and breeding of Campanula.