核桃青皮的化学成分研究——酚类化合物

核桃青皮是一种传统的中药材,含有大量的酚类化合物,具有镇痛、消炎、抑菌、抗肿瘤等功效。为了从核桃青皮中分离得到更多的酚类成分,以利于更好地阐明其作用机理,该研究采用大孔树脂Diaion HP-20SS、凝胶Sephadex LH-20,HPLC等方法对核桃青皮80%的乙醇提取物进行分离纯化。结果表明:共分离了10个单体化合物,它们的结构经质谱(MS)、一维核磁共振谱(~1H NMR和~(13)C NMR)、二维核磁共振谱(HSQC,HMBC)数据的分析及文献数据的比较确定为没食子酸(1),没食子酸甲酯(2),对羟基苯甲酸(3),3,4-二羟基苯甲酸甲酯(4),6-O-咖啡酸-D-葡萄糖(5),6-O-没食子酸-葡萄糖苷(6),4,8-二羟基-1-四氢萘醌(7),5,8-二羟基-4-甲氧基-1-四氢萘酮(8),5,8-二羟基-1-四氢萘酮(9),4-羟基-1-四氢萘酮(10)。其中,化合物5,化合物6为属内首次分离到。该研究结果为进一步深入研究核桃青皮的化学成分和药理作用提供了一定参考。

Chemical constituents from walnut green husk: Phenols

Walnut green husk, a traditional Chinese herbal medicine, is often used for analgesic, anti-inflammatory, antibacterial, anti-tumor and so on. Previous studies show that walnut green husk contains lots of phenolic compounds. In order to get more monomer compounds and to facilitate better study of its physiological mechanism, 80% ethanol extract of walnut green husk was separated by the chromatography(macroporous resin HP-20SS, Sepadexs LH-20, and HPLC)and ten compounds were obtained. Their structures were identified by MS,1D(¹H and ¹³C)NMR, 2D(HSQC, HMBC)NMR spectral data analyses, as well as comparison with reported data as:gallic acid(1), methyl gallate(2), p-hydroxybenzoic acid(3), methyl-3,4-dihydroxybenzoate(4), 6-O-caffeic acid-D-glucose(5), 6-O-gallicacid-glucoside(6), 4,8-dihydroxy(7), 4,5,8-trihydroxy-1-tetralone(8), 5,8-dihydroxy-1-tetralone(9), 4-hydroxy-1-tetralone(10). Compounds 5 and 6 were isolated from the genus Juglans for the first time. The results can provide a reference for further study on the chemical composition and pharmacological effects of walnut green husk.