| HPLC法测定牡荆中牡荆素的含量 |
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| 引用本文： | 罗娅君,边清泉,陈 佳,蒋云福,陈 驹.HPLC法测定牡荆中牡荆素的含量[J].广西植物,2011,31(3):418-421. |
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| 作者姓名： | 罗娅君 边清泉 陈 佳 蒋云福 陈 驹 |
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| 作者单位： | 绵阳师范学院,化学与化学工程学院,四川,绵阳,621000 |
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| 基金项目： | 四川省教育厅重点项目，绵阳师范学院科研项目 |
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| 摘 要： | 建立了牡荆中牡荆素含量的高效液相色谱测定方法,对牡荆资源进行评价,为牡荆的质量标准的建立提供参考.样品采用超声波提取法提取,色谱柱为菲罗门柱(5 μm,250 mm×4.6 mm),流动相为甲醇-水溶液(4∶6),流速为1.0 mLmin-1,检测波长为340 nm,进样量为5 μL,在柱温30 ℃时对牡荆中牡荆素的含...
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| 关 键 词： | 超声提取 高效液相色谱 牡荆 含量测定 |
Determination of vitexin in Vitex negundo
var.cannabifolia by HPLC |
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| LUO Ya-Jun,BIAN Qing-Quan,CHEN Ji,JIANG Yun-Fu,CHEN Ju.Determination of vitexin in Vitex negundo
var.cannabifolia by HPLC[J].Guihaia,2011,31(3):418-421. |
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| Authors: | LUO Ya-Jun BIAN Qing-Quan CHEN Ji JIANG Yun-Fu CHEN Ju |
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| Institution: | College of Chemistry & Chemical Engineering, Mianyang Normal University, Mianyang 621000, China |
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| Abstract: | A method was established for determining contents of vitexin in Vitex negundo var.cannabifolia(Sieb.et Zucc.)by HPLC in order to evaluate this resource.Vitexin was obtained by ultrasonic extraction,separated on Filo door column(5 μm,250 mm×4.6 mm)with a mixture of methanol-water(volume ratio 4∶6)as mobile phase at flow rate 1.0 mL·min-1.The UV detective wavelength was 340 nm,injection volume was 5 μL,and the column temperature was set at 30 ℃.Vitexin content showed a good linear relationship within 0.04520-0.4068 μg.(r=0.9998) and the average recovery was 100.4%,RSD was 1.63%.This method is simple,accurate and can be used for quality control of Vitex negundo var.cannabifolia. |
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| Keywords: | ultrasonic eextraction HPLC Vitex negundo var cannabifolia determination |
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