| 甜荞查尔酮合酶基因Chs的克隆及序列分析 |
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| 引用本文： | 李成磊,张晓伟,吴 琦,赵海霞,陈 惠,邵继荣.甜荞查尔酮合酶基因Chs的克隆及序列分析[J].广西植物,2011,31(3):383-387. |
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| 作者姓名： | 李成磊 张晓伟 吴 琦 赵海霞 陈 惠 邵继荣 |
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| 作者单位： | 四川农业大学,生命科学与理学院,四川,雅安,625014 |
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| 摘 要： | 利用RT-PCR技术从甜荞中克隆得到查耳酮合酶(CHS)的cDNA开放阅读框(ORF)序列,命名为FeChs,NCBI登录号为GU172166.1.该序列长1 179 bp,编码392个氨基酸,与其它植物CHS基因的同源性为78%～92%,其推导的氨基酸序列含有CHS高度保守的活性位点及CHS的标签序列GFGPG.
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| 关 键 词： | 甜荞 查耳酮合成酶基因 克隆 序列分析 |
Cloning and sequence analysis of chalcone synthase
gene Chs from Fagopyrum esculentum |
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| LI Cheng-Lei,ZHANG Xiao-Wei,WU Qi,ZHAO Hai-Xi,CHEN Hui,SHAO Ji-Rong.Cloning and sequence analysis of chalcone synthase
gene Chs from Fagopyrum esculentum[J].Guihaia,2011,31(3):383-387. |
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| Authors: | LI Cheng-Lei ZHANG Xiao-Wei WU Qi ZHAO Hai-Xi CHEN Hui SHAO Ji-Rong |
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| Institution: | College of Life Science, Sichuan Agricultural University, Ya'an 625014, China |
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| Abstract: | The cDNA ORF sequence of chalcone synthase gene(FeChs)was amplified by RT-PCR from Fagopyrum esculentum.Its accession number is GU172166.1 on GenBank.The anlyasis indicated the cDNA sequence was 1 179 bp,encoding 392 amino acids and had a homlogy 78%-92% with other plants'CHS genes.The deduced amino acids sequence of FeChs contained the highly conserved catalytic active sites,and the typical Tag sequence of CHS with GFGPG. |
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| Keywords: | Fagopyrum esculentum chalcone synthase gene cloning sequence analysis |
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