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| 梨蒴珠藓愈伤组织诱导和配子体再生研究 | |
| 引用本文： | 付素静,方炎明,张启香.梨蒴珠藓愈伤组织诱导和配子体再生研究[J].广西植物,2011,31(3):403-406. |
| 作者姓名： | 付素静 方炎明 张启香 |
| 作者单位： | 1. 贵州省铜仁学院,生化系,贵州,铜仁,554300;南京林业大学,森林资源与环境学院,南京,210037 2. 南京林业大学,森林资源与环境学院,南京,210037 3. 浙江林学院,林业与生物技术学院,浙江,临安,311300 |
| 摘 要： | 以梨蒴珠藓无菌藓株为外植体诱导愈伤组织和配子体再生,接种于含不同激素组合的MS和Knop固体培养基上,分别进行愈伤组织和不定芽的分化,并探讨愈伤组织诱导和配子体再生的适宜培养条件.结果显示,愈伤组织诱导的最佳培养基是MS+0.5 mg/L BA+0.1 mg/L 2,4-D,愈伤组织诱导率为33.3%;不定芽诱导的最佳... |
| 关 键 词： | 梨朔珠藓 组织培养 愈伤组织 配子体再生 |
Studies on callus induction and gametophyte regeneration of Bartramia pomiformis |
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| FU Su-Jing,FANG Yan-Ming,ZHANG Qi-Xiang.Studies on callus induction and gametophyte regeneration of Bartramia pomiformis[J].Guihaia,2011,31(3):403-406. | |
| Authors: | FU Su-Jing FANG Yan-Ming ZHANG Qi-Xiang |
| Institution: | 1.Department of Biology and Chemistry, Tongren University, Tongren 554300, China; 2.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China; 3.School of Forestry and Biotechnology, Zhejiang Forestry College, Lin'an 311300, China |
| Abstract: | This research was conducted to select a suitable culture system for the callus induction and gametophyte regeneration of Bartramia pomiformis.And axenic mosses were inoculated on MS and Knop solid media with different hormone combilations for callus and buds induction and differentiation.The result showed that MS medium containing 0.5 mg/L BA,0.1 mg/L 2,4-D was optimal for callus induction,with the induction rate of 33.3%.Optimal medium for inducing gametophyte is MS+1.0 mg/L BA,and the achieved multiplication coefficient was 10.0.And Knop with no hormone was as well as MS in generation of gametophyte.This investigation could provide some important references for callus induction and systematics of B.pomiformis. |
| Keywords: | Bartramia pomiformis in vitro culture callus gametophyte regeneration |
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