不同成花量金花茶花果期果枝叶内源激素的变化

该研究采用ISSR分子标记,对黄枝油杉7个自然种群的遗传多样性进行了分析。结果表明：用12条ISSR引物对218个黄枝油杉个体进行扩增,共扩增出125个位点。在物种水平上,多态性位点百分数( PPL)为100.00％,Shannon信息多样性指数( I)为0.4177,Nei’ s基因多样性指数( H)为0.2666；在种群水平上,多态性位点百分数(PPL)在71.20％~92.00％之间,平均值为80.69％,Shannon信息多样性指数(I)在0.3273~0.3886之间,平均值为0.3548,Nei’ s基因多样性指数( H)在0.2139~0.2478之间,平均值为0.2291。这说明黄枝油杉在物种水平和种群水平上均显示出较高的遗传多样性。 Nei’ s遗传多样性分析( Gst＝0.1433)和AMOVA分析(Φst＝17.91％)表明,黄枝油杉的遗传变异主要存在于种群内,种群间的遗传分化程度较低,种群间保持一定的基因交流( Nm＝2.9890>1)。 Mantel分析显示,黄枝油杉种群间的遗传距离和地理距离之间不存在显著的相关关系( r＝0.4567, P＝0.0610>0.05)。

Dynamic changes of endogenous hormones in fruit branch leaves of Camellia nitidissima with different flower numbers during blossom and fruit periods

In order to investigate the physical problem of flowering and fructification rate of endangered plant Camellia nitidissima, and provide the theory basis for species conservation, the contents and ratio of GA₃, IAA, ZR and ABA in fruit branch leaves of C. nitidissima of more-flower, less-flower and non-flower were measured by ELISA(Enzyme-linked immunosorbent assay)method during blossom and fruit periods. In early-bloom stage, the content of IAA in leaves rised firstly and then fell, and its content of more-flower-plant was lower than that of less-flower-plant leaves; the content of ZR in flower-plant was higher than that of non-flower-plant; the content of GA₃ appeared an upward trend, and its content of flower-plant was higher than non-flower-plant; the contents of ABA decreased firstly and then increased, and its content of more-flower-plant was lower than non-flower-plant. In full-bloom stage, the contents of IAA, GA₃ and ABA declined overall; the content of ZR rised firstly and then fell; the contents of IAA, ZR, GA₃ in more-flower plant were higher than those in less-flower-plant or non-flower-plant, while the content of ABA was less than that of other plants. During the bloom period, the ratio of IAA/ZR, IAA/ABA, ZR/ABA and GA₃/ABA of flower-plant were higher than that of non-flower-plant, but the ratio of(IAA+GA₃)/ZR was just in contrast, which also indicate the development of flower and fruit was related to not only single plant hormone but also the balance of plant hormones. During autumn shoot period with active vegetative growth, the ratio of IAA/ZR of non-flower-plant was larger than that of flower-plant, while the ratio of IAA/ZR of non-flower-plant was smaller in blossom and fruit period with vigorous reproductive growth, but the ratio of(IAA+GA₃)/ZR was just in contrast. The results showed that high level of IAA, ABA, ZR and low level of GA₃ in bud stage was benefitial for blossom, high content of IAA, ZR and low content of GA₃, ABA in post-bloom stage was conducive to reducing blossom and fruit dropping, increased fruit-bearing rate and promoting fruit rapid growth, high ABA content before harvesting was favorable toward fruit ripening. Therefore, it can provide theoretical reference for using plant growth regulator to reduce the fallen petal and fruit abscission in production of C. nitidissima.