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| 激素对樱桃番茄两种外植体诱导再生植株的影响 | |
| 引用本文： | 魏琴,周黎军,周锦霞,曹有龙,陈放.激素对樱桃番茄两种外植体诱导再生植株的影响[J].广西植物,2002,22(5):441-T008. |
| 作者姓名： | 魏琴 周黎军 周锦霞 曹有龙 陈放 |
| 作者单位： | 1. 四川大学生命科学学院,四川,成都,610064 2. 宜宾学院生物工程系,四川,宜宾,644007 3. 宁夏农业重点实验室,宁夏,银川,750002 |
| 摘 要： | 以 MS为基本培养基 ,附加不同浓度的 6-BA、IAA和 NAA培养樱桃番茄两种外植体以诱导再生植株。结果表明 :含 NAA的 MS+6-BA2 mg/L(单位下同 ) +NAA0 .2培养基诱导的叶片愈伤组织 ,经继续培养无芽的分化 ,含 IAA的 MS+6-BA2 +IAA0 .2培养基诱导的愈伤组织 ,经继续培养诱导芽的分化 ;含 NAA或IAA的 MS+6-BA2 +NAA0 .2和 MS+6-BA2 +IAA0 .2培养基利于下胚轴愈伤组织的诱导 ,而不含生长素的 MS+6-BA1 .0培养基可直接诱导芽的分化。 |
| 关 键 词： | 激素 樱桃番茄 再生植株 |
| 文章编号： | 1000-3142(2002)05-0441-03 |
Effects of phytohormones on regeneration from two explants in Lycopersicum esculentum |
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| WEI Qin,ZHOU Li-jun,ZHOU Jin-xia,CAO You-long,CHEN Fang.Effects of phytohormones on regeneration from two explants in Lycopersicum esculentum[J].Guihaia,2002,22(5):441-T008. | |
| Authors: | WEI Qin ZHOU Li-jun ZHOU Jin-xia CAO You-long CHEN Fang |
| Institution: | WEI Qin1,ZHOU Li-jun3,ZHOU Jin-xia3,CAO You-long2,CHEN Fang1 |
| Abstract: | Two explants from Lycopersicum esculentum were cultured on MS medium supplemented with 6-BA,IAA and NAA of different density to induce regeneration plants. The results showed that the callus from leaf which has been induced on medium MS+6-BA2 mg/L(Unit is the same as follows)+NAA0.2 is not differentiated into bud by subculture, and on medium MS+6-BA2+IAA0.2 it can be differentiated into bud by subculture. It is favorable to induce callus of hypocotyl on medium MS+6-BA2.0+NAA0.2 and MS+6-BA2.0+IAA0.2, and bud may be directly induced with hypocotyl on medium MS+6-BA1.0. |
| Keywords: | phytohormone Lycopersicum esculentum Mill var cerasiforme ssp Subpont aneum regeneration plant |
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