植物原生质体在分子细胞生物学研究中的应用

植物原生质体是去除了细胞壁的裸露细胞,其具有细胞全能性,现广泛应用于植物分子细胞生物学的研究中,可以大大缩减实验周期,并有助于得到体内实验的实时检测数据。该文除了介绍植物原生质体的提取和纯化方法外,还对国内外利用各种植物的原生质体进行细胞瞬时转化、亚细胞定位、细胞融合和大分子复合物相互作用等试验进行了总结和讨论。植物原生质体还可用于基因表达模式的实时检测,并作为生物反应器的受体细胞进行代谢物的体外生产。此外,还对当前该技术所面临的瓶颈进行了分析,为植物原生质体在分子细胞生物学领域的应用提供帮助,为技术的优化和推广提供参考。

Application of plant protoplasts in molecular and cell biology research

Plant protoplasts are naked cells without cell walls. They have been extensively applied in the researches of plant molecular and cell biology for their totipotency, which could greatly shorten the experimental periods and help to get massive effective and real-time experimental detection data in vivo. In this article, in addition to introduce the purification of plant protoplasts, we mainly summarized the application of plant protoplasts in the respects of transient transformation, subcellular localization, cell fusion and macromolecular complex interaction. Plant protoplasts could also be used to survey the expression pattern of gene in real-time detection, as well as the target cells for the production of metabolites in bioreactors. Furthermore, we have compared the advantages and disadvantages of plant protoplasts in the current research, which provides new insights into the researches on plant molecular and cell biology. We have also analyzed the difficulties in the application of plant protoplasts, which provides the reference for the optimization and promotion of this technology.