脱水与复水过程中湿地匍灯藓的生理生化响应

研究了脱水与复水过程中湿地匍灯藓的渗透调节能力、抗氧化保护系统以及DNA损伤与修复的影响。结果表明:(1)在脱水过程中,游离脯氨酸、可溶性糖、还原性糖含量均出现明显增加,脱水12h时达到最大;细胞膜透性上升;超氧化物歧化酶、过氧化氢酶和过氧化物酶活性均持续上升,抗坏血酸含量则逐渐降低;DNA损伤明显加大,脱水24h时DNA已全部降解为低分子量片段。(2)在复水过程中,以上各项指标变化趋势与脱水处理时刚好相反。表明湿地匍灯藓具备复苏植物的典型特征,在含水量变化(变水)过程中具有较强的抗干(旱)性,其抗逆性主要体现为修复能力。

Physiological response of Plagiomnium acutum during desiccation and rehydration process

Ability of osmo-regulation,changes of protective enzymes activities and ascorbic acid content and DNA damage/repair were studied in Plagiomnium acutum during desiccation and rehydration process.Results showed that:(1)on the desiccation phase,content of proline,soluble sugar and reducing sugar increased obviously,and got the peak at 12 h;peameability of membrane raised;activities of protective enzymes,such as SOD,CAT and POD,increased continually,thus the content of antixidative substrate AsA decreased gradually at the same time;DNA degradated gradually,only some low molecular weight framents existed at the end.(2)all the indexes mentioned above showed an opposite tendency on the rehydration phase.Based on these,it can be concluded that P.acutum has the typical characteristic of resurrection plant,possess a strong ability on anti-dry(drought)during the variation of moisture content,and it mainly attributes to the ability of repairing.