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| 农杆菌介导红江橙遗传转化的研究初报 | |
| 引用本文： | 贺红,韩美丽,李耿光.农杆菌介导红江橙遗传转化的研究初报[J].广西植物,2002,22(3):256-258. |
| 作者姓名： | 贺红 韩美丽 李耿光 |
| 作者单位： | 1. 广州中医药大学中药学院,广东广州,510405 2. 广西林业科学院,广西南宁,530001 3. 中国科学院华南植物研究所,广东广州,510650 |
| 基金项目： | 国家自然科学基金项目 (编号 :39870 5 39) |
| 摘 要： | 用红江橙实生苗的上胚轴为材料 ,初步研究以根癌农杆菌介导的 GUS基因转化。结果表明 :以卡那霉素作为选择试剂进行选择培养时 ,Km浓度为 5 0 mg/L;外植体以平放为好 ;抑菌剂选择头孢霉素较好。GUS基因瞬时表达检测 ,70 .4%的外植体呈阳性反应 ;GUS基因稳定表达检测 ,在获得的 1 2株抗性植株中 ,GUS反应呈阳性所占比例为 1 6.7%。 |
| 关 键 词： | 红江橙 农杆菌 遗传转化 |
| 文章编号： | 1000-3142(2002)03-0256-03 |
| 修稿时间： | 2000年12月4日 |
Preliminary study on the Agrobacterium-mediated genetic transformation of Citrus sinensis Osbeck cv. Hongjiang |
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| HE Hong ,HAN Mei li ,LI Geng guang.Preliminary study on the Agrobacterium-mediated genetic transformation of Citrus sinensis Osbeck cv. Hongjiang[J].Guihaia,2002,22(3):256-258. | |
| Authors: | HE Hong HAN Mei li LI Geng guang |
| Institution: | HE Hong 1,HAN Mei li 2,LI Geng guang 3 |
| Abstract: | The transformation of GUS gene by Agrobacterium mediated was studied. The explants used for transformation were the epicotyls from Citrus sinensis Osbeck cv. Hongjiang . The results indicated that the concentration of kanamycin used as the reagent of selection was 50 mg/L; when the explants were cultured on the selection medium, they placed horizontally better than they oriented with their apical ends protruding from the medium. Cefotaxime used as antibiotics was better than carbenicillin. Histochemical GUS assay showed that 70 4% of the explants had the transient expression of GUS gene and 16 7% of the 12 resistant plants were GUS positive. |
| Keywords: | Citrus sinensis Osbeck cv Hongjiang Agrobacterium genetic transformation |
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