高压静电对烟草愈伤组织生长和根分化的效应

用场强 +1.0 KV/ cm高压静电 ( HV EF)处理烟草愈伤组织 30 min,处理组愈伤组织生长量达 10 .6 4g,比对照组高 8.0 % ,根分化率达 70 .6 % ,比对照组高 95%。超氧物歧化酶 ( SOD)活性、蛋白质含量分别为356 .3U/ g.FW、11.0 7m g/ g.FW,分别比对照增加 10 .5%、2 3.5%。过氧化物酶 ( POD)和吲哚乙酸 ( IAA)氧化酶活性处理组分别为 56 .3OD4 70 / min.g.FW、87.1μg/ g.h.FW,分别比对照下降 2 6 .1%、8.7%。结果表明 ,高压静电处理可促进烟草愈伤组织细胞生长和根的分化 ,这与 HV EF影响体内 SOD、POD和 IAA氧化酶活性以及蛋白质含量的变化相关。

The effect of high voltage electrostatic field (HVEF)on growth and root differentiation of Nicotiana tabacum L. Callus

The Callus of Nicotiana tabacum L. with 30 bottles per treatment was treated by the high voltage electrostatic field(HVEF) at+1.0/cm for 30 min. The treated callus were cultured at 28 °C chamber temperature with 10 h of light,1 800 lx to 2 000 lx of illumination eath day for 40 days. The results showed that the callus growth in treated group was 10.64 g,which was 8.0% higher than that of control. The root differentiation rate was 70.6%,which was 95% higher than that of control. The activity of SOD,content of protein in the treated group were 356.3 U/g.Fw and 11.07 mg/g.Fw respectively,which were 10.5% and 23.5% higher than those of control respectively. The activities of peroxidase and IAA oxidase were 56.3 OD470/min.gFW,87.1 ug/h.gFW respectively,which were 26.1%,and 8.7% respectively lower than those control. According to the results we could concluded that HVEF promoted the growth and the root differentiation of Nicotiana tabacum L. callus. This result may be caused by the effects of HVEF on the activities SOD and POD and IAAoxidase,and the content of protein in Nicotana tabacum L. callus.