| 高效液相色谱法测定银杏叶提取物中黄酮甙含量 |
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| 引用本文： | 李典鹏, 方宏,.高效液相色谱法测定银杏叶提取物中黄酮甙含量[J].广西植物,1996,16(3):292-294. |
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| 作者姓名： | 李典鹏 方宏 |
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| 作者单位： | 广西植物研究所 |
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| 摘 要： | 用高效液相色谱法测定银杏叶提取物经酸水解后黄酮甙元含量．采用Ｃ１８柱，甲醇：水：磷酸（５５：４４．５：０．５）为流动相，检测波长３７０ｎｍ，方法回收率９５．１％～１０３．２％，变异系数（ＣＶ）３．１７％，１０批样品含测平均为２５．４％．
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| 关 键 词： | 高效液相色谱 银杏叶黄酮甙 槲皮素 银杏叶提取物 |
DETERMINATION OF FLAVONOIDS IN EXTRACT OF GINKGO BILOBA LEAVES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) |
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| Li Dianpeng,Fang Hong.DETERMINATION OF FLAVONOIDS IN EXTRACT OF GINKGO BILOBA LEAVES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)[J].Guihaia,1996,16(3):292-294. |
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| Authors: | Li Dianpeng Fang Hong |
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| Abstract: | A HPLC method has been developed for the determination of Flavonoids in extract of Ginkgo biloba, leaves. (EGb). Procedure includes hydrolpsis of flavonoids and subsequent quantitative chromatographic assay of the obtained aglycones on a C18 colunm using methanol, water and phosphoric acid (55: 44.5: 0.5) as mobile phase, and UV detection at 370 mm. The results have shown that the recoveries were between 95.1%-103.2% and CV was 3.17%. The average content of ginkgo flavanol glycosides in EGb was 25.4%. |
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| Keywords: | HPLC flavanol glycosides quercetin extract of Ginkgo biloba |
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