超富集植物对重金属耐受和富集机制的研究进展闫 研 李建平 赵志国 林庆宇(505) 狭叶香蒲吸收Cd2+的动力学特征

采用水培方法,加入Cd2+浓度为0、0.2、0.4、0.6、1、1.2、1.5mg·L-1,分别在4、8、12、24h研究狭叶香蒲幼苗吸收Cd2+的动力学特征。结果表明,狭叶香蒲对Cd2+的吸收基本符合Michaelis-Menten动力学模型。在上述几个时间段中,以0～4h时间段内狭叶香蒲对Cd2+吸收的最大吸收速率Vmax最大,Km最小,α值也最大,此时根系吸收能力最强。随时间的延长(4～24h),Vmax变小,但是净吸收速率V仍在增加。用V-C作图法和L-B作图法求解动力学参数结果差异不大,且两种方法曲线模拟的相关性均较好。

The kinetics characteristic of Cd2+ absorption of Typha angustifolia

Cd2+ concentration of 0,0.2,0.4,0.6,1.0,1.2,and 1.5 mg·L-1were added in hydroponic culture solution to Typha angustifolia plants,and the Kinetics characteristic of Cd2+ absorption of them were studied separately at 4 h,8 h,12 h,and 24 h.Results indicated that the Cd2+ absorption of T.angustifolia conformed to Michaelis-Menten Kinetics model in the main.Among the before time periods,in 0-4 h time period,the maximum absorption rate Vmax of T.angustifolia was the highest,the Km was the lowest,and the α value was the highest,it showed that the root absorption ability was the strongest.With the time extending(4-24 h),the Vmax decreased,but the completely absorption rate still increased.The kinetic parameters value obtained by V-C construction method and L-B construction method differed insignificantly,and the relativity of the curve imitated by the two methods all were good.