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| 罗汉果SRAP反应体系的建立与优化 | |
| 引用本文： | 刘丽华,马小军,孙晶晶,覃嘉明,魏建和.罗汉果SRAP反应体系的建立与优化[J].广西植物,2009,29(6):894-898. |
| 作者姓名： | 刘丽华 马小军 孙晶晶 覃嘉明 魏建和 |
| 作者单位： | 1. 中国医学科学院,北京协和医学院,药用植物研究所,北京,100193 2. 辽宁工程技术大学,理学院,阜新,123000 3. 广西玉米研究所,南宁,530227 |
| 基金项目： | 国家科技支撑计划课题(2006BA109B01-07) |
| 摘 要： | 建立适合罗汉果的SRAP-PCR扩增体系,为罗汉果的遗传图谱构建及基因定位奠定基础。实验对罗汉果SRAP-PCR反应体系的影响因素(引物,dNTP,Taq酶,Mg~(2+),模板DNA)在多个水平上进行优化试验,筛选出各反应因素的最佳水平,建立了罗汉果SRAP-PCR反应的最佳体系(10μL):引物0.6μmol/L、dNTP0.25 mmol/L、Taq DNA聚合酶0.5U、Mg~(2+)2.0 mmol/L和模板DNA 30 ng。该体系的建立能很好的满足罗汉果基因组DNA的扩增要求,SRAP标记应用于罗汉果遗传研究是可行的。 |
| 关 键 词： | 罗汉果 SRAP-PCR 反应体系 建立 |
Establishment and optimization of SRAP reaction system in Siraitia grosvenorii |
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| LIU Li-Hu,MA Xiao-Jun,SUN Jing-Jing,QIN Jia-Ming,WEI Jian-He.Establishment and optimization of SRAP reaction system in Siraitia grosvenorii[J].Guihaia,2009,29(6):894-898. | |
| Authors: | LIU Li-Hu MA Xiao-Jun SUN Jing-Jing QIN Jia-Ming WEI Jian-He |
| Institution: | 1.Institute of Medicinal Plant Development, China Academy Medicinal Science, Chinese Peking Union Medical Colledge, Beijing 100193, China; 2.College of Science, Liaoning Technical University, Fuxin 123000, China; 3.Guangxi Maize Research Institute, Nanning 530227, China |
| Abstract: | The design was used to establish SRAP-PCR amplification system on Siraitia grosvenorii DNA so as to lay foundation for genetic map construction and gene mapping. The factors influencing SRAP analysis,including primers,dNTP,Taq polymerase,Mg~(2+),and the concentration of DNA template were studied in a number of levels respectively. The results showed that: 0.6 μmol/L primer,0.25 mmol/L dNTP,0.5U Taq DNA polymerase,2.0 mmol/L Mg~(2+) and 30 ng DNA template in 10 μL reaction system were the best suitable SRAP-PCR system for Siraitia grosvenorii. It was feasible to apply SRAP marker in genetic research in Siraitia grosvenorii. |
| Keywords: | SRAP-PCR Siraitia grosvenorii SRAP-PCR reaction system establishment |
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