波斯小麦醇溶蛋白遗传多样性分析

应用APAGE技术对来源于16个国家(地区)96份波斯小麦材料进行醇溶蛋白遗传多样性分析,结果表明,波斯小麦具有丰富的醇溶蛋白等位变异,共分离出55条迁移率不同的带纹。每份材料可电泳出13～26条,平均18.6条,带纹多态性为100%。96份材料共出现了75种电泳图谱类型,其中31份材料共同拥有10种图谱,剩余的65份材料的电泳图谱各不相同。供试材料间GS值变异范围为0.176～1.000,平均值为0.538。聚类分析表明,在GS值0.538水平上供试材料可聚为五大类群,且遗传聚类关系与材料的地理来源有一定的相关性。

Genetic diversity of gliadin in Triticum carthlicum

Genetic variations of gliadins among 96 Triticum carthlicum accessions from 16 countries were evaluated by APAGE. Higher genetic variability was observed. A total of 55 polymorphism bands were separated, and 75 gliadin patterns were found, among which 31 accessions shared 10 gliadin patterns and 65 accessions had unique gliadin patterns. The mean genetic similarity (GS) was 0.538, ranging from 0.176 to 1.000. All accessions were clustered into 5 groups. The clustering analysis suggested that the genetic relationship among T.carthlicum accessions based on the gliadins were associated with its geographical distribution.