激素对洋桔梗植株再生的影响及生根培养的研究

以MS为基本培养基 ,附加不同浓度的 6 BA、KT、NAA和IBA诱导洋桔梗叶片外植体的再生植株。结果表明 :MS +6 BA 0 .5～ 1 .0mg/L(单位下同 ) +NAA 0 .2和MS +6 BA 0 .5～ 1 .0 +IBA 0 .2培养基都能诱导外植体产生愈伤组织 ,但 6 BA的浓度必须小于 1 .0mg/L ,否则会导致组织的严重玻璃化 ;MS +KT 1 .0～2 .0 +NAA 0 .2或MS +KT 1 .0～ 2 .0 +IBA 0 .2培养基也能诱导外植体产生愈伤组织 ,愈伤组织出现的时间较早且质地较好 ,适合分化。继代培养时 ,MS培养基中仅加 6 BA 0 .5mg/L或KT 0 .5mg/L ,即能获得较高的分化率。生根培养研究中 ,培养液为 1 /2MS+5 0g/L糖 +IBA 2mg/L的前处理 ,生根效果较好 ,生根率接近基质生根培养的生根率。

Effect of hormone on plantlet regeneration and studies of root regeneration culture of Eustoma sp.

The leaves explants from Eustoma sp. were cultured on MS medium supplemented with 6-BA,KT,NAA and IBA of different densities to induce regeneration plants. The results indicated that the callus can be induced on all medium of MS+6-BA 0.5～1.0 mg/L(Unit is the same as follows)+NAA 0.2 and MS+6-BA 0.5～1.0+IBA 0.2,but the density of 6-BA can not exceed 1.0 mg/L,otherwise,it will cause serious vitrification;At the same time,on these medium of MS+KT 1.0～2.0+NAA 0.2 or MS+KT 1.0～2.0+IBA 0.2,callus can also be induced early and its texture is better for differentiation. When subculturing,MS medium only supplemented with 6-BA 0.5 or KT 0.5 can obtain higher frequency of differentiation. When studying root regeneration culture,the explants that has been pretreated with culture liquid of 1/2 MS+50 g/L sucrose+IBA 2 mg/L had better root regeneration and root regeneration frequency was close to medium culture’s.