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厚壳桂新微卫星体分子标记的开发与应用
引用本文:高丹丹,王峥峰,朱 鹏,叶万辉.厚壳桂新微卫星体分子标记的开发与应用[J].广西植物,2011,31(1):39-42.
作者姓名:高丹丹  王峥峰  朱 鹏  叶万辉
作者单位:1. 中国科学院,植物资源保护和可持续利用重点实验室,中国科学院,华南植物园,广州,510650;广东省数字植物园重点实验室,中国科学院,华南植物园,广州,510650;中国科学院,研究生院,北京,100049
2. 中国科学院,植物资源保护和可持续利用重点实验室,中国科学院,华南植物园,广州,510650;广东省数字植物园重点实验室,中国科学院,华南植物园,广州,510650
基金项目:国家重点基础研究"973"发展计划项目,国家科技支撑计划项目,中国科学院知识创新工程重大项目,"十一五"国家科技支撑计划项目
摘    要:生境破碎化是导致全球生物多样性危机的主要原因,即使常见物种也会受到很大影响.厚壳桂(Cryptocarya chinensis)是我国南亚热带季风常绿阔叶林群落演替顶极物种,由于这一森林植被的破坏而呈片断化分布.在以往的研究中,我们利用筛选到的一些微卫星体对其遗传多样性研究后发现其可能以无性生殖为主.为进一步证实这一发...

关 键 词:无性生殖  保育  森林片断化  遗传标记

Isolation and characterization of new set of microsatellite loci in Cryptocarya chinensis
GAO Dan-Dan,WANG Zheng-Feng,ZHU Peng,YE Wan-Hui.Isolation and characterization of new set of microsatellite loci in Cryptocarya chinensis[J].Guihaia,2011,31(1):39-42.
Authors:GAO Dan-Dan  WANG Zheng-Feng  ZHU Peng  YE Wan-Hui
Institution:1.Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; 2.Guangdong Key Laboratory of Digital Botanical Garden, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; 3.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
Abstract:Habitat fragmentation is the main threat to global biodiversity. Common species could also greatly suffer from habitat fragmentation. As a result of the destruction of the monsoon evergreen broad-leaved forest in South China,Cryptocarya chinensis,one of the climax species,is now patchily distributed. Because a few microsatellite loci reported previously might cause low resolution to detect asexual reproduction in this species,we reported a new set of microsatellite which will assist to detect the extensiveness of sexual and asexual reproduction at fine spatial scale in the future. Eleven microsatellite markers from repetitive DNA enriched libraries for C.chinensis were developed. Twenty-one individuals from Dinghu Mountain in South China were used to characterize their polymorphism. The number of alleles of 11 loci ranged from two to three,observed and expected heterozygosities ranged from 0.048 to 1.000,and 0.048 to 0.535,respectively. Deviations from Hardy-Weinberg equilibrium were detected in 8 loci due to heterozygote excess (highly significant negative Fis). All locus pairs showed significant linkage disequilibrium at the 0.05 significant level,but such significance disappeared after Bonferroni correction. 20 of 21 detected individuals showed identical multilocus genotype. All these results conformed to the genetic characteristic of clonal species,confirming extensive clonal growth in C.chinensis.
Keywords:asexual reproduction  conservation  forest fragmentation  genetic marker
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