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芦荟属植物种间杂交及其F_1代POD同工酶鉴定
引用本文:铁军,金山,白海艳,刘瑞祥,吴志萍.芦荟属植物种间杂交及其F_1代POD同工酶鉴定[J].广西植物,2005,25(5):449-452,i0008.
作者姓名:铁军  金山  白海艳  刘瑞祥  吴志萍
作者单位:1. 长治学院生化系,山西长治,046011
2. 内蒙古农业大学林学院,内蒙古呼和浩特,010019
摘    要:以芦荟属植物元江芦荟和皂质芦荟为亲本进行远缘有性杂交试验。正交对3株103朵小花进行授粉,获得7枚果实,平均结实率为6.80%;反交对2株88朵小花授粉,获得5枚果实,平均结实率为5.88%。获得了19株F1代实生苗,并对亲本和F1代实生苗以及库拉索芦荟、华芦荟等5种芦荟属植物进行了过氧化物酶同工酶(POD)的比较研究,结果显示,5种芦荟种间的POD同工酶酶谱具有较高的相似程度,同时各种又具有各自的特征酶带,很容易区分。特别是F1代实生苗的酶谱与父母本的酶谱有显著不同的酶带,证明产生了新的中间类型。

关 键 词:芦荟属  种间杂交  F1代实生苗  POD同工酶
文章编号:1000-3142(2005)05-0449-04
收稿时间:2004-07-19
修稿时间:2004-07-192005-01-18

The interspecific hybridization and identification of F1 hybrids by POD isozyme application in Aloe
TIE Jun,JIN Shan,BAI Hai-yan,LIU Rui-xiang,WU Zhi-ping.The interspecific hybridization and identification of F1 hybrids by POD isozyme application in Aloe[J].Guihaia,2005,25(5):449-452,i0008.
Authors:TIE Jun  JIN Shan  BAI Hai-yan  LIU Rui-xiang  WU Zhi-ping
Abstract:In the paper,we described a distant sexual hybridization experiment in which 19 F1 seedlings were generated from maternal plant Aloe yuanjiangensis Xiong,Zheng et Liu of Aloe L.and paternal plant A.saponaria (Ait.)Haw.7 fruits were obtained from direct cross,average fructicative rate was 6.80%;5 fruits were obtained from reciprocal cross,average rate was 5.88%. By the comparing research of peroxide isoenzyme(POD)on the 5 species of plants of Aloe L.:the paternal and maternal plants,F1 seedlings, A.vera L., A.chinensis (Haw.)Baker,etc. It is easy to distinguish the respective characteristic enzyme zones of the 5 species of plants of Aloe L.although their enzyme spectrums are rather similar. Particularly,the distinctive differences between F1 seedlings and their paternal and maternal plants proved that the new intermediate types were generated.
Keywords:Aloe  interspecific hybridization  F1 seedling  POD isozyme
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