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桂南地区苦玄参药材RP-HPLC指纹图谱研究
引用本文:梁小燕,方,宏,宁德生,陈海珊,黄永林.桂南地区苦玄参药材RP-HPLC指纹图谱研究[J].广西植物,2007,27(6):948-952.
作者姓名:梁小燕      宁德生  陈海珊  黄永林
作者单位:广西壮族自治区,中国科学院,广西植物研究所,广西,桂林,541006
基金项目:广西自然科学基金(0339079)~~
摘    要:采用反相HPLC法测定了广西梧州、龙州、苍梧、越南等多产地12批苦玄参药材指纹图谱,并对不同产地的苦玄参指纹图谱进行比较。色谱条件为:LunaC18(4.6×250mm,5μm)色谱柱,乙腈-水梯度洗脱,流速1.0mL/min,检测波长254nm,柱温25℃。结果12批苦玄参样品指纹图谱共标定了16个分离度良好的共有峰,方法的精密度、稳定性、重复性均符合国家相关规定,可作为控制苦玄参药材质量的定性标准。

关 键 词:苦玄参  HPLC  指纹图谱  质量控制
文章编号:1000-3142(2007)06-0948-04
收稿时间:2006-12-11
修稿时间:2007-06-19

RP-HPLC fingerprint of Picria fel-terrae from South Guangxi
LIANG Xiao-Yan,FANG Hong,NING De-Sheng,CHEN Hai-Shan,HUANG Yong-Lin.RP-HPLC fingerprint of Picria fel-terrae from South Guangxi[J].Guihaia,2007,27(6):948-952.
Authors:LIANG Xiao-Yan  FANG Hong  NING De-Sheng  CHEN Hai-Shan  HUANG Yong-Lin
Institution: Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China
Abstract:The RP-HPLC assay was used to establish the fingerprint of Picria fel-terrae from South Guangxi,and the HPLC chromatogram of different origins of Picria fel-terrae were compared.The chromatography conditions were as follows:Luna C18 column(4.6×250 mm,5 μm),a mixture of acetonitrile and water as mobile phase in gradient mode,flow rate was 1.0 mL/min,detective wavelength at 254 nm,column temperature 25℃.The fingerprints of P.fel-terrae with 16 common peaks were determined.The RSD of precision and reproducibility lay within 5%.According to this method,the established fingerprint can be used for the identification and quality control of P.fel-terrae.
Keywords:Picria fel-terrae  HPLC  fingerprint  quality control
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