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外部因子对蝴蝶兰叶片原球茎状体发生的影响
引用本文:杨美纯, 周歧伟, 许鸿源, 卢美英,.外部因子对蝴蝶兰叶片原球茎状体发生的影响[J].广西植物,2000,20(1):42-46.
作者姓名:杨美纯  周歧伟  许鸿源  卢美英  
作者单位:广西大学农学院,广西南宁,530005
摘    要:对影响蝴蝶兰叶片原球茎状体 (PL B,Protocorm - like- body)发生和植株再生的外部因子进行了研究。结果表明 :BA是决定原球茎状体发生的主要因子 ;苹果汁、香蕉汁和椰子汁明显促进原球茎状体的形成 ;在 MS+BA5mg/L+椰子汁 15%的培养基中 ,蝴蝶兰叶片原球茎状体的诱导率可达 6 0 %以上 ;活性炭可有效防止外植体叶块变褐死亡 ;多效唑 1.5mg/L可促进再生植株根的形成 ,使叶片变厚变短变绿 ,使试管苗移栽成活率达 95%以上

关 键 词:蝴蝶兰  组织培养  原球茎状体  植株再生

Effect of external factors on protocorm-like-body inducement in Phalaenopsis leaves
YANG Mei-chun,ZHOU Qi-wei,XU Hong-yuan,LU Mei-ying.Effect of external factors on protocorm-like-body inducement in Phalaenopsis leaves[J].Guihaia,2000,20(1):42-46.
Authors:YANG Mei-chun  ZHOU Qi-wei  XU Hong-yuan  LU Mei-ying
Abstract:WT5”BZ] The effect of external factors on protocorm like body(PLB) inducement and plant regeneration in Phalaenopsis were studied. The results indicated that BA was the most important external factor for determining PLB development;Apple juice、banana juice and coconut juice promoted PLB inducement; when BA 5 mg/L and 15% coconut juice were added into MS medium,the inducing rate of the PLB from leaves was more than 60%. Active cardon was effective for preventing the explant from browning and dying, MET 1.5 mg/L promoted the development of regenerated plant root, and the survival rate of transplants was more than 95%. WT5”HZ]
Keywords:Phalaenopsis  tissue culture  protocorm  like  body  plant regeneration
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