迁地保护的金花茶遗传多样性评价

采用ISSR分子标记技术对迁地保护的金花茶3个人工栽培居群进行遗传多样性分析。揭示了迁地保护金花茶的遗传多样性水平和结构。11条引物共扩增出87条带,其中36条具有多态性,多态位点比例(P)为41.38%。单个居群的多态带百分率P从21.84%到25.29%,平均值为24.14%。在物种水平上,期望杂合度和Shannon信息指数分别为0.1525和0.2273;在居群水平上:期望杂合度和Shannon信息指数分别为0.1069和0.1528。通过与野生居群遗传参数比较,表明该种质圃基本有效地保护金花茶的遗传多样性总水平。遗传分化系数为0.2990。表明迁地保护金花茶遗传变异发生在居群内的个体间占70.10%、29.90%的遗传变异发生在居群间。

Genetic diversity evaluation of ex-situ populations of Camellia nitidssima, detected by ISSR markers

Genetic diversity of 3 ex-situ population of Camellia nitidssima was detected by ISSR markers. 87 bands were generated by 11 ISSR primer,of which 41.38% bands were polymorphic. The percentage of polymorphic bands(P)was from 21.84% to 25.29%,24.14% on average at the population level. Expected heterozygosity(Ht)and Shannon’s information(Ho)were 0.152 5 and 0.227 3 respectively at specific level,0.106 9 and 0.152 8 at population level. Compared with the parameters form natural population,the germplasm nursery maintained most genetic diversity of Camellia nitidssima. More than 70% of genetic diversity resided among individuals within population.