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Isolation and characterization of 39 microsatellite loci in the endangered Japanese loach Leptobotia curta |
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| Authors: | Watanabe Katsutoshi Takeshima Hirohiko Iwata Akihisa Abe Tsukasa Uehara Kazuhiko Kakioka Ryo Kihira Daijiro Nishida Mutsumi |
| Institution: | Division of Biological Science, Graduate School of Science, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo, Kyoto 606-8502, Japan, Ocean Research Institute, University of Tokyo, 1-15-1 Minamidai, Nakano, Tokyo 164-8639, Japan, Division of Southeast Asian Area Studies, Graduate School of Asian and African Area Studies, Kyoto University, 46 Yoshida-Shimoadachicho, Sakyo, Kyoto 606-8501, Japan, Ushimado Marine Laboratory, Graduate School of Natural Science and Technology, Okayama University, Ushimado, Setouchi, Okayama 701-4303, Japan, Aquatic Life Conservation Research Center, Research Institute of Environment, Agriculture and Fisheries, Osaka Prefectural Government, Neyagawa, Osaka 572-0088, Japan, Aquatic Life Conservation Society, 802-5-10 Nihonbashi-Tomizawacho, Chuo, Tokyo 103-0006, Japan. |
| Abstract: | A microsatellite-enriched genomic library was obtained for the endangered Japanese loach Leptobotia curta, and 39 dinucleotide markers were successfully isolated and characterized. These markers had between one and nine alleles, with expected heterozygosity ranging from 0 to 0.839, in a population from the Lake Biwa-Yodo River system of Japan. Linkage equilibrium was observed in most loci, and only one locus showed significant deviation from Hardy-Weinberg equilibrium. These microsatellite markers will be useful for genetic diversity studies of wild and captive L. curta populations. |
| Keywords: | Botiinae Cobitidae kissing loach STR |
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