PERMANENT GENETIC RESOURCES: Isolation of microsatellite loci from the endangered plant Sibara filifolia (Brassicaceae) |
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| Authors: | McGlaughlin Mitchell E Wallace Lisa E Helenurm Kaius |
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| Institution: | Department of Biology, University of South Dakota, CL 191, 414 East Clark Street, Vermillion, SD 57069, USA Department of Biological Sciences, Mississippi State University, Mississippi State, MS 39762, USA. |
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| Abstract: | Sibara filifolia (Brassicaceae) is a federally endangered annual herb found on two of the California Channel Islands. Previous studies based on allozymes revealed little genetic variability on San Clemente Island. Nine polymorphic microsatellite loci were isolated from individuals on San Clemente Island. We found low levels of allelic variation (mean NA = 2.3), with seven loci exhibiting significant deviations from Hardy–Weinberg equilibrium (P < 0.01) and 10 pairs of loci exhibiting significant linkage disequilibrium (P < 0.01). Most of the observed variability (mean HO = 0.003) occurred among populations or in rare homozygous individuals. |
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| Keywords: | California Channel Islands conservation genetics microsatellite Sibara |
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