Isolation of novel microsatellites from Jatropha curcas L. and their cross-species amplification |
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Authors: | Pamidimarri D V N Sudheer Sinha Ramini Kothari Pragya Reddy Muppala P |
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Institution: | Discipline of Wasteland Research, Central Salt and Marine Chemical Research Institute (CSIR), G-B Marg. Bhavnagar 364002, Gujarat, India. |
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Abstract: | Jatropha curcas L., a member of the Euphorbiaceae, is widely distributed in different parts of the globe. In the present study, 12 microsatellites were isolated from J. curcas and their cross-species amplification was checked in six species of genus Jatropha. Within J. curcas, observed and expected heterozygosities ranged from 0.94 to 0.54 and from 0.95 to 0.56, respectively. Of the 12 loci, five showed significant deviation from Hardy-Weinberg equilibrium. There was no significant linkage disequilibrium detected between any of the loci. The markers isolated in the present investigation will be useful for assessing the population diversity and genetic structure of J. curcas and also in other species of Jatropha. |
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Keywords: | biodiesel cross‐species amplification FIASCO (fast isolation by AFLP of sequences containing repeats) Jatropha curcas microsatellites |
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