In vitro rearing ofExorista larvarum on tissue culture-based diets |
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Authors: | Alessandro Bratti Amadou Konoti Coulibaly |
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Institution: | (1) Istituto di Entomologia ‘Guido Grandi’, University of Bologna, Via F. Re 6, 40126 Bologna, Italy |
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Abstract: | Exorista (=Tachina) larvarum (L.) (Diptera, Tachinidae), a polyphagous parasitoid that attacksLymantria dispar L. andHyphantria cunea (Drury), was rearedin vitro from egg to adult on four tissue culture media-based diets (TMM-FH, SCHNEIDER'S, EX-CELL 400, and SF-900). The kind of tissue
culture media in the diets did not influence the adult yield (34 to 55%) and puparium weight (26–27 mg). Adult yield and the
puparium weight ofE. larvarum developed on TNM-FH and SCHNEIDER'S-based diets containing different amounts ofGalleria mellonella pupal extract (PE) (0, 1.25, 2.5 and 5%), were lower on diets without PE. In diets without PE development times from oviposition
to adult emergence, were shorter on TNM-FH (19 days) than on SCHNEIDER'S-based diet (25–26 days). The adults that developed
on artificial diets were able to parasitize the factitious hostG. mellonella and produce viable progeny. The results demonstrate thatE. larvarum is the most promising parasitoid ever studied forin vitro mass production. |
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Keywords: | Exorista larvarum parasitoids Tachinidae Lymantria dispar Hyphantria cunea Galleria mellonella artificial diet in vitro rearing |
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