Schistosoma mansoni: the IMP4 gene is involved in DNA repair/tolerance after treatment with alkylating agent methyl methane sulfonate |
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Authors: | Furtado Carolina Regis-da-Silva Carlos Gustavo Passos-Silva Danielle Gomes Franco Glória Regina Macedo Andréa Mara Junho Pena Sérgio Danilo Machado Carlos Renato |
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Institution: | Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, CP 486, 31270-010 Belo Horizonte, Minas Gerais, Brazil. |
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Abstract: | Using a functional complementation strategy, we have isolated a Schistosoma mansoni cDNA that complemented Escherichia coli mutant strains which are defective in the DNA base excision repair pathway. This cDNA partially complemented the MMS-sensitive phenotype of these strains. The sequence of the isolated cDNA was homologous to genes involved in the RNA metabolism pathway, especially ScIMP4 of Saccharomyces cerevisiae. To establish whether the S. mansoni cDNA clone could complement yeast ScIMP4-defective mutants, we constructed a yeast haploid strain that coded for a truncated Imp4p protein. This mutant strain was treated with different DNA damaging agents, but showed only MMS sensitivity. The functional homology between the ScIMP4 gene and the cDNA from S. mansoni was verified by partial complementation of the mutant yeast with the worm's gene. This gene appears to be involved in DNA repair and RNA metabolism in both S. mansoni and S. cerevisiae. |
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Keywords: | Schistosoma mansoni DNA repair IMP4 RNA metabolism BER base excision repair cDNA complementary DNA DNA deoxyribonucleic acid LB Luria-Bertani MGMT 6-methyl guanine methyl transferase MMS methyl methane sulfonate ORF open reading frame PCR polymerase chain reaction PEG polyethylene glycol RNA ribonucleic acid rRNA ribosomal RNA RNP ribonucleic protein snoRNA small nucleolar RNA snoRNP small nucleolar RNP ssDNA single-stranded DNA UV ultraviolet YPD yeast extract/peptone/dextrose YNB yeast nitrogen base |
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