| 子宫内膜异位细胞改良体外培养技术的研究进展 |
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| 引用本文： | 黄秋月,蔡三金,付先芸,庄明燕,王薇.子宫内膜异位细胞改良体外培养技术的研究进展[J].生命的化学,2021(1):77-83. |
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| 作者姓名： | 黄秋月 蔡三金 付先芸 庄明燕 王薇 |
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| 作者单位： | 三峡大学医学院;三峡大学妇女儿童医院;三峡大学中医临床医院 |
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| 基金项目： | 国家自然科学基金面上项目(81973897);湖北省卫生厅科研一般项目(WJ2019F079);宜昌市医疗卫生科研项目(A19-301-44)。 |
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| 摘 要： | 体外细胞培养是子宫内膜异位性疾病研究的重要工具。本文回顾了细胞体外培养技术在分离、纯化环节的改良以及子宫内膜异位性疾病永生化细胞系改造中的研究进展,并总结了近几年来激素及细胞因子诱导培养、细胞共培养及三维培养技术在该疾病体外机制研究中的应用,并对其发展前景进行了展望。
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| 关 键 词： | 子宫内膜异位性疾病 永生化细胞系 诱导培养 三维培养 |
Development of in-vitro cell culture technique in endometriosis |
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| HUANG Qiuyue,CAI Sanjin,FU Xianyun,ZHUANG Mingyan,WANG Wei.Development of in-vitro cell culture technique in endometriosis[J].Chemistry of Life,2021(1):77-83. |
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| Authors: | HUANG Qiuyue CAI Sanjin FU Xianyun ZHUANG Mingyan WANG Wei |
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| Institution: | (Medical College,China Three Gorges University,Yichang 443002,China;Women and Children's Hospital of China Three Gorges University,Yichang 443000,China;Clinical Hospital of Traditional Chinese Medicine,China Three Gorges University,Yichang 443002,China) |
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| Abstract: | Cell culture in vitro is an important tool in the study of endometriosis. In this paper, the research progress of cell culture technology in isolation, purification and the modification of cell line is reviewed. The application of hormone and cytokine induced culture, cell co-culture and three dimensional culture technology in the in vitro mechanism research of endometriosis are summarized, and its development prospect is discussed. |
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| Keywords: | endometriosis immortalized cell line inducing culture three-dimensional culture |
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