| 卵母细胞的生发泡移植 |
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| 引用本文： | 征月良.卵母细胞的生发泡移植[J].生命的化学,2011(3). |
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| 作者姓名： | 征月良 |
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| 作者单位： | 临沂师范学院生命科学学院; |
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| 摘 要： | 生发泡(germinal vesicle,GV)移植到去核的GV期卵母细胞后,获得重构卵,重构卵在体外能成熟,受精和进行胚胎发育。GV移植到去核的第二次减数分裂中期(metaphase Ⅱ,MII)卵母细胞后,重构卵能发生GV破裂,但难以排出第一极体。GV移植后,通过连续核移植,重构合子具有发育到终期的能力。GV移植为研究卵母细胞的发育提供了一种重要工具。
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| 关 键 词： | 卵母细胞 生发泡 移植 |
Germinal vesicle transfer of oocytes |
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| ZHENG Yueliang College of Life Science,Linyi Normal University,Linyi ,China.Germinal vesicle transfer of oocytes[J].Chemistry of Life,2011(3). |
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| Authors: | ZHENG Yueliang College of Life Science Linyi Normal University Linyi China |
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| Institution: | ZHENG Yueliang College of Life Science,Linyi Normal University,Linyi 276000,China |
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| Abstract: | Germinal vesicles(GVs) are transferred into enucleated GV stage oocytes,and the reconstructed oocytes are able to undergo maturation,fertilization and embryonic development in vitro.When GVs are transferred into enucleated metaphase II oocytes,some reconstructed oocytes undergo GVBD but none extrudes a first polar body.Multiple nuclear transfers are performed after GV transfer,and the reconstructed zygotes have the ability to support embryonic development to term.GV transfer is an important tool to study th... |
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| Keywords: | oocyte germinal vesicle transfer |
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