人类蛋白质相互作用组

高通量酵母双杂交与免疫亲和纯化技术的快速发展和日臻成熟,使得在蛋白质组水平上大规模地研究蛋白质之间的相互作用成为可能。目前,人类蛋白质互作网络在细胞、组织、器官乃至整个个体水平的研究已经陆续展开。蛋白质互作网络中蛋白质数量也由少数几个向整个蛋白质组扩展。同时,功能、疾病、生态等相关的蛋白质互作网络研究也取得了一定的成果。然而,人类的蛋白质互作网络研究正面临着一些问题和挑战。本文综述了人类蛋白质互作网络的研究方法、研究进展以及面临的挑战,同时指出了人类蛋白质互作网络研究的方向和目标。

Human protein-protein interactome

The rapid development of yeast two-hybrid and immuno-affinity purification make the study of protein-protein interaction in a large scale at the proteome level possible. At present, studies on human protein-protein interaction network in cell, tissue, organ, and even whole body have been doing successively, and the protein number in the protein-protein interaction network have increased from several to whole proteome. The studies of protein-protein interaction network about function, disease and ecology have made some achievements. However, there are also some questions and challenges in the present studY. This paper reviews the research methods of the protein- protein interaction network, the research progress and challenges, and points out the orientation and target of research of the human protein-protein interaction network.