Hyperbolic regression analysis for kinetics, electrophoresis, ELISA, RIA, Bradford, Lowry, and other applications |
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Authors: | Studnicka Gary M |
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Institution: | International Genetic Engineering, Inc. 1545 Seventeenth Street, Santa Monica, CA 90404, USA |
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Abstract: | Hyperbolic regression analysis is an effective method for fittingexperimental data points obtained from a variety of experimentsin molecular biology, including enzyme kinetics, agarose gelelectrophoresis of DNA fragments, SDSpolyacrylamide gelelectrophoresis of proteins, enzymelinked immunosorbentassays (ELISA), radioimmunoassays (RIA), Bradford protein quantitationassays, Lowry protein assays, and other applications. Hyperbolicregression yields excellent fitted curves without the biasesthat are introduced by carrying out linear regression on doublereciprocal coordinates, and it produces one simple equation,encompassing all the data points, that can be used easily ina pocket calculator to estimate the values of unknown samplesfrom the known standards.
Received on February 9, 1986; accepted on April 11, 1986 |
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