Sexual mating of Botrytis cinerea illustrates PRP8 intein HEG activity |
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| Authors: | Annika AM Bokor Jan AL van Kan Russell TM Poulter |
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| Institution: | 1. Biochemistry Department, University of Otago, 710 Cumberland Street, Dunedin 9054, New Zealand;2. Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands;1. Department of Surgery, University of South Alabama College of Medicine, Mobile, Alabama;2. Department of Pharmacology, University of South Alabama College of Medicine, Mobile, Alabama;3. Center for Lung Biology, University of South Alabama College of Medicine, Mobile, Alabama;4. University of South Alabama College of Medicine, Mobile, Alabama;1. Institute of Food and Agricultural Technology- XaRTA-CIDSAV, University of Girona, C/ Maria Aurèlia Capmany, 61. 17071 Girona, Spain;2. Departamento de Microbiología, Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, IHSM-UMA-CSIC, Universidad de Málaga, Facultad de Ciencias, Campus de Teatinos s/n. 29071 Málaga, Spain;1. Department of Chemistry Education, Korea National University of Education, Chungbuk 28173, South Korea;2. Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejoen 34141, South Korea;1. Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil;2. Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil |
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| Abstract: | Strains of Botrytis cinerea are polymorphic for the presence of an intein in the Prp8 gene (intein +/?). The intein encodes a homing endonuclease (HEG). During meiosis in an intein +/? heterozygote, the homing endonuclease initiates intein ‘homing’ by inducing gene conversion. In such meioses, the homing endonuclease triggers gene conversion of the intein together with its flanking sequences into the empty allele. The efficiency of gene conversion of the intein was found to be 100%. The extent of flanking sequence affected by the gene conversion varied in different meioses. A survey of the inteins and flanking sequences of a group B. cinerea isolates indicates that there are two distinct variants of the intein both of which have active HEGs. The survey also suggests that the intein has been actively homing during the evolution of the species and that the PRP8 intein may have entered the species by horizontal transfer. |
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