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| 2个油菜CMS系统的酯酶和过氧化物酶同工酶分析 | |
| 引用本文： | 朱彦涛,张新,刘湛,李殿荣,郭蔼光.2个油菜CMS系统的酯酶和过氧化物酶同工酶分析[J].西北植物学报,2009,29(4). |
| 作者姓名： | 朱彦涛 张新 刘湛 李殿荣 郭蔼光 |
| 作者单位： | 1. 西北农林科技大学,生命科学学院,陕西省农业分子生物学重点实验室,陕西杨陵,712100;国家油料作物改良中心陕西油菜分中心 2. 国家油料作物改良中心陕西油菜分中心 3. 西北农林科技大学,生命科学学院,陕西省农业分子生物学重点实验室,陕西杨陵,712100 |
| 基金项目： | 国家重点基础研究发展规划(973计划) |
| 摘 要： | 以甘蓝型油菜细胞质雄性不育系(CMS)Polima A及其保持系Polima B、陕2A及其保持系陕2B 4个材料初花期的叶片、叶柄以及花蕾组织为材料,采用聚丙烯酰胺垂直板凝胶电泳比较它们在酯酶(EST)和过氧化物酶(POD)同工酶酶谱的差异.结果表明,甘蓝型油菜不育系与其对应保持系的叶片、叶柄的EST同工酶谱均无明显差异,但两系花蕾的EST同工酶谱有一定的差异;Polima A与陕2A对应器官的EST同工酶谱均表现出明显差异.不育系与其对应保持系的叶片、叶柄的POD同工酶谱差异不明显,而两系花蕾的POD同工酶谱有明显的差异;两个不育系之间的POD同工酶谱明显不同.花蕾的EST和POD同工酶的条带数目明显多于相应材料的叶片和叶柄,且EST同工酶的条带数目明显多于相应的POD同工酶.因此,甘蓝型油菜CMS系统Polima A和陕2A有着不同的遗传背景. |
| 关 键 词： | 甘蓝型油菜 细胞质雄性不育系 保持系 酯酶 过氧化物酶 聚丙烯酰胺凝胶电泳 |
Study on Esterase and Peroxidase Isozymograms of Two Cytoplasmic Male Sterility Systems in Brassica napus L. |
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| ZHU Yan-tao,ZHANG Xin,LIU Zhan,LI Dian-rong,GUO Ai-guang.Study on Esterase and Peroxidase Isozymograms of Two Cytoplasmic Male Sterility Systems in Brassica napus L.[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(4). | |
| Authors: | ZHU Yan-tao ZHANG Xin LIU Zhan LI Dian-rong GUO Ai-guang |
| Institution: | 1 College of Life Science;Key Laboratory of Plant Molecular Biology of Shaanxi Province;Northwest A&F University;Yangling;Shaanxi 712100;China;2 Rapeseed Branch of Shaanxi Province;National Oil-Crop Improvement Center;Hybrid Rapeseed Research Center of Shaanxi Province;Dali;Shaanxi 715105;China |
| Abstract: | The polyacrylamide vertical board gel electrophoresis of Esterase(EST) and Peroxidase(POD) isozymograms were carried out with the leaf blade,petiole as well as flower bud organization of 4 materials including PolimaA and its maintainer line PolimaB,Shaan2A and its maintainer line Shaan2B in the early flowering season in Brassica napus L.The results showed that between the cytoplasmic male sterility(CMS) lines and their corresponding maintainer lines,EST isozymograms of leaf and petiole had not distinct diff... |
| Keywords: | Brassica napus L cytoplasmic male sterility line maintainer line esterase peroxidase polyacrylamide gel electrophoresis |
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