首页 | 本学科首页   官方微博 | 高级检索  
   检索      

辣椒种质遗传多样性的RAPD和ISSR及其表型数据分析
引用本文:陈学军,程志芳,陈劲枫,娄群峰,耿红.辣椒种质遗传多样性的RAPD和ISSR及其表型数据分析[J].西北植物学报,2007,27(4):662-670.
作者姓名:陈学军  程志芳  陈劲枫  娄群峰  耿红
作者单位:1. 作物遗传与种质创新国家重点实验室,南京农业大学园艺学院,南京,210095;江西省农业科学院蔬菜花卉研究所,南昌,330200
2. 作物遗传与种质创新国家重点实验室,南京农业大学园艺学院,南京,210095
基金项目:国家高技术研究发展计划(863计划);国家重点实验室基金
摘    要:用RAPDI、SSR分子标记及28个表型性状数据对辣椒属5个栽培种的13份材料进行了分析,结果表明:23条RAPD引物共扩增出209条带,平均每个引物扩增出9.09条,多态性位点比率为83.73%;16条ISSR引物共扩增出94条带,平均每个引物扩增出5.88条,多态性位点比率为79.79%.与RAPD相比,ISSR标记检测到的有效等位基因数(Ne)及Shannon多样性指数(I)、遗传离散度(Ht)和遗传分化系数(Gst)等遗传多样性参数都较大,多态性位点比例在亲缘关系较近的一年生辣椒(Capsicum annuum)种内较高,说明ISSR有更高的多态性检测效率,并且适合亲缘关系较近的种群间遗传多样性分析.基于RAPDI、SSR的聚类与基于表型数据的聚类之间存在极显著正相关,且都能将C.annuum与其它栽培种区分开来.

关 键 词:辣椒  遗传多样性  表型性状
文章编号:1000-4025(2007)04-0662-09
修稿时间:2006-11-15

Genetic Diversity of Pepper Germplasm with RAPD,ISSR and Phenotypic Data
CHEN Xue-jun,CHENG Zhi-fang,CHEN Jin-feng,LOU Qun-feng,GENG Hong.Genetic Diversity of Pepper Germplasm with RAPD,ISSR and Phenotypic Data[J].Acta Botanica Boreali-Occidentalia Sinica,2007,27(4):662-670.
Authors:CHEN Xue-jun  CHENG Zhi-fang  CHEN Jin-feng  LOU Qun-feng  GENG Hong
Institution:1 .National Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Hortieulture,Nanjing Agricultural University,Nanjing 210095,China;2. Vegetable and Flower Institute,Jiangxi Academy of Agricultural Seienees,Nanehang 330200,China
Abstract:Thirteen accessions from five cultivated Capsicum species were analyzed with RAPD and ISSR markers,and 28 phenotypic traits.Twenty-three RAPD primers and sixteen ISSR primers generated 209 and 94 fragments,respectively.The number of fragments per primer,the percentage of polymorphic loci was in turn 9.09,83.73% for RAPD markers,and 5.88,79.79% for ISSR markers.But ISSR had higher percentage of polymorphic loci in C.annuum than RAPD,and effective number of alleles(Ne),Shannon's Information index(I),heterozygosity(Ht),the genetic differentiation coefficient(Gst) based on ISSR markers were bigger than those based on RAPD as well.The results showed that ISSR markers could detect more genetic diversity in the pepper accessions and be more suitable for assessment of genetic diversity among accessions that have higher consanguinity levels.Three dendrograms for the 13 accessions were formed based on RAPD,ISSR-generated bands and phenotypic data.The correlation analysis among genetic similarities based on RAPD,ISSR markers and phenotypic data indicated they were significantly correlated.All the dendrograms could separate C.annuum from the other cultivated Capsicum species.
Keywords:RAPD  ISSR
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《西北植物学报》浏览原始摘要信息
点击此处可从《西北植物学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号