拟南芥表皮毛发育突变体abt3-1的基因定位及表型分析

在发掘和鉴定调控植物表皮毛发育的新因子过程中,获得了一个表皮毛发育异常的拟南芥隐性突变体abt3-1(aberrantly branched trichome 3-1)。与野生型拟南芥(Col-0)相比,其表皮毛分支数目明显增加。另外,abt3-1还表现出植株小、叶形宽、叶色发灰、主根短等发育缺陷。利用图位克隆技术将该突变基因ABT3定位在1号染色体上,分子标记在F28G11#3与F4N21#1之间,物理距离为134kb。该研究将为进一步克隆ABT3基因及研究其在调控植物生长发育过程中的作用奠定基础。

Gene Mappingand Phenotype Analyses of an Arabidopsis thaliana Trichome Developmental Mutant abt3 1

In the process of discovering and identifying new factors that regulate the development of plant trichome, we obtained a recessive Arabidopsis thaliana mutant aberrantly branched trichome 3 1 (abt3 1).Compared to the wild type (Col 0)A. thaliana, abt3 1 appeared a high proportion of multi branched trichomes.In addition, abt3 1 is dramatically reduced in size and has a defective primary root, wide shaped grey leaves. The mutant gene ABT3 was precisely mapped into the 134 kb chromosome interval between the molecular markers F28G11#3 and F4N21#1 on chromosome 1, which was determined using the position cloning strategy.This research will lay a foundation for further cloning ABT3 gene and studying its role in the regulation of plant growth and development.