利用流式细胞光度术鉴定苹果倍性的研究

利用流式细胞光度术测定了苹果１２个二倍体，５个三倍体细胞ＤＮＡ含量。结果表明：二倍体细胞核ＤＮＡ含量平均为２．２７ｐｇ，三倍体细胞核ＤＮＡ含量平均为３．１３ｐｇ。

USE OF FLOW COTOMETRY FOR IDENTIFICATION OF PLOIDY IN APPLE

Content of nuclear DNA for apple cultivars including 12 diploid and 5 triploid was measured by flow cytometry.The average nuclear DNA content of diploid cultivars was 2.27 pg and that of triploid cultivars was 3.13 pg,which indicated that variation of nuclear DNA content between diploid and triploid was significant.Nuclear DNA content of 6 colchicine induced plants were also measured.That these induced plants had two groups of cells which nuclear DNA content diverged almost one times,which suggested that these inducing plant were chimerals.The result shows that flow cytometry could be used for identifying ploidy.Compared to count chromosome number for identifying ploidy,using flow cytometry for identification of ploidy was ideal method because of simple preparation of sample,rapid and accurate measurement.