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太白米组织培养的研究
引用本文:赵银萍 王Zhe之 等.太白米组织培养的研究[J].西北植物学报,2003,23(2):339-341.
作者姓名:赵银萍  王Zhe之
作者单位:陕西师范大学生命科学学院,西安,710062
基金项目:教育部重点课题,陕西省自然科学基金
摘    要:太白米地下鳞茎在MS附加不同浓度KT、BA、IAA、NAA、2,4-D的培养基上,可诱导产生愈伤组织,其中在MS附加NAA 0.5mg/L,KT0.1mg/L的培养基上愈伤组织诱导率最高,可达65%,且生长快;培养在MS附加2,4-D 1.0mg/L,KT 0.1mg/L培养基上的鳞茎可经不定根直接发育成新的鳞茎,由此建立了太白米的鳞茎再生体系,鳞茎再生率达2.17倍。

关 键 词:太白米  鳞茎  组织培养
文章编号:1000-4025(2003)02-0339-03
修稿时间:2002年4月15日

Study on the tissue culture of Notholirion bulbuliferum
ZHAO Yin-ping,WANG Zhe-zhi,CAO Xiao-yan.Study on the tissue culture of Notholirion bulbuliferum[J].Acta Botanica Boreali-Occidentalia Sinica,2003,23(2):339-341.
Authors:ZHAO Yin-ping  WANG Zhe-zhi  CAO Xiao-yan
Abstract:The tissue culture and plant regeneration of notholirion bulbuliferum were studied in this paper and the result showed that;the calli could be induced from bulbule on the MS basicl mediums supplemented with different concentration of KT,BA,IAA,NAA and 2,4-D. The induction rate of calli were highest and reached to 65% on the medium of MS+NAA 0. 5 mg/L+KT 0. 1 mg/L. The bulbules which were cultured on MS + 2.4-D 1. 0 mg/L+KT 0. 1 mg/L medium could bring up new bulbules by adventitious root. The regeneration rate of bulbules was as high as 2. 17 times and the regeneration system were built.
Keywords:Notholirion bulbuliferum  bulbule  tissue culture
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