5个银杏优良品种成熟胚离体繁殖培养基的选择研究

选用5个优良银杏品种的成熟胚,分别接种在B5、WPM、DCR3种基本培养基上进行试管培养.结果表明: 1 不同银杏品种在不同的培养基上培养28d后,成苗率、根长、苗高出现差异.方差分析表明,培养基之间对银杏成熟胚离体培养的差异大于品种之间的差异; 2 DCR培养基对5个品种的成苗率、苗高和根长无显著性差异,是最适宜的培养基,WPM是较好的培养基,B5是稍差的培养基. 3 从本批次5个品种的优良度上比较,依次为44号>45号>28号>79号>53号.

In vitro cultural medium selection for the mature embryos of five elite cultivars of Ginkgo biloba

In this paper,we compared the growth index of the mature embryos of five cultivars of Ginkgo biloba L.in three basic media (B_5,WPM,DCR).ANOVA analysis indicated that the different among media is more significant than that of among cultivars.No significant difference were detected among the rate of normal seedling,seedling height and root length of the five tested cultivars in the DCR medium,therefore the DCR medium was suggested as the most suitable medium for culturing the mature embryos of Ginkgo biloba WPM medium was suggested as the second better medium,B_5 medium was little suitable medium.The sequence of seed goodness of the five elite cultivars was No.44>No.45>No.28>No.79>No.53.