低温对螺旋藻可溶性蛋白和游离氨基酸外渗的影响

以鄂尔多斯高原碱湖特有的钝顶螺旋藻S1、引进的钝顶螺旋藻S2和极大螺旋藻S3为材料，采用Folin法和茚三酮法分别测定低温下细胞外渗液中可溶性蛋白和游离氮基酸含量。结果表明：处理温度越低，时间越长，螺旋藻细胞外渗液中可溶性蛋白和游离氨基酸的含量越高。S1膜损伤程度最轻，比引进种S2、S3有较强的抗寒能力。判断低温对螺旋藻膜损伤的程度，用可溶性蛋白较游离氨基酸更接近于实际。

Effect of Low Temperature on the Exosmosises of Soluble Protein and Free Amino Acid from Spirulina (Arthrospira)

Folin procedure and ninhydrin method were adopted to determine the contents of soluble proteins and free amino acids in the cellular extravasations of Spirulina (Arthrospira) platensis (S_1) only found in the natron lakes of the Ordos Plateau,introduced S. (A.) platensis (S_2) and S. (A.) maxima (S_3) .The results showed that the lower the treating temperature was and the longer the treating time lasted,the higher the contents of soluble protein and free amino acids in the cellular extravasation of Spirulina (Arthrospira) would become.S_1 suffered the least membrane damage,which indicated that it had a stronger frost resistance than S_2 and S_3.The damage of low temperature to Spirulina (Arthrospira) membrane that was evaluated according to soluble proteins would be closer to its ;ractical damage than that according to free amino acids.