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桤叶唐棣组织培养研究
引用本文:杜保国,杨途熙,魏安智,杨锋利.桤叶唐棣组织培养研究[J].西北植物学报,2005,25(2):400-404.
作者姓名:杜保国  杨途熙  魏安智  杨锋利
作者单位:西北农林科技大学,林学院,陕西杨陵,712100
摘    要:以桤叶唐棣的带芽茎段为外植体,对桤叶唐棣离体培养技术进行了初步研究,结果表明:初代培养基为MS IAA0.1mg/L 6-BA 0.5mg/L KT 0.5mg/L;增殖培养基为MS IAA 0.1mg/L 6-BA 2.0mg/L,增殖芽数最大,达到5.4;GA3对茎芽长无促进作用;在1/4MS无激素培养基上,生根率达87.9%。

关 键 词:桤叶唐棣  组织培养  快速繁殖
文章编号:1000-4025(2005)02-0400-05

Tissue Culture of Amelanchier alnifolia
DU Bao-guo,YANG Tu-xi,WEI An-zhi,YANG Feng-li.Tissue Culture of Amelanchier alnifolia[J].Acta Botanica Boreali-Occidentalia Sinica,2005,25(2):400-404.
Authors:DU Bao-guo  YANG Tu-xi  WEI An-zhi  YANG Feng-li
Abstract:In order to study the best method for tissue culture and rapid propagation for Amelanchier alnifolia Nutt., stems with buds were chosen as explants. The results of in vitro culture showed that the shoot differentiation medium was MS+IAA 0.1 mg/L+6-BA 0.5 mg/L+KT 0.5 mg/L,the optimum medium for proliferation was MS+IAA 0.1 mg/L+6-BA 2.0 mg/L, GA_3 has no positive effect on the growth of shoot, the best medium for rooting was 1/4 MS without hormones, the rooting rate reached 87.9%.
Keywords:Amelanchier alnifolia Nutt    tissue culture  rapid propagation
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