|
|||||
|
|
| S-腺苷甲硫氨酸代谢途径相关基因在小麦水分胁迫中的表达 | |
| 引用本文： | 李昌澎,周琳璘,陈亮,黄林周,陈晓杰,王宇坤,胡银岗.S-腺苷甲硫氨酸代谢途径相关基因在小麦水分胁迫中的表达[J].西北植物学报,2011,31(6):1120-1126. |
| 作者姓名： | 李昌澎 周琳璘 陈亮 黄林周 陈晓杰 王宇坤 胡银岗 |
| 作者单位： | 1. 西北农林科技大学动物科技学院,陕西杨陵,712100 2. 西北农林科技大学农学院,陕西杨陵,712100 3. 西北农林科技大学动物科技学院,陕西杨陵712100;西北农林科技大学农学院,陕西杨陵712100;国家小麦改良中心杨凌分中心,陕西杨陵,712100;陕西省农业分子生物学重点实验室,陕西杨陵712100 |
| 基金项目： | '863'重点项目子课题，科技部973计划前期项目，高等学校学科创新引智计划项目，澳大利亚ACIAR项目 |
| 摘 要： | 以小麦品种‘晋麦47’为材料,利用半定量RT-PCR方法,对S-腺苷甲硫氨酸代谢途径中的S-腺苷甲硫氨酸合成酶(SAMS)基因、S-腺苷甲硫氨酸脱羧酶(SAMDC)基因和γ-谷氨酰半胱氨酸合成酶(-γECS)基因在正常供水、PEG-6000模拟水分胁迫和复水过程中小麦叶片的表达模式进行了分析。结果表明,3个基因在正常生长情况下有一定量的表达,SAMS和SAMDC基因在水分胁迫早期(PEG-6000胁迫6、12、244、8 h)上调表达,水分胁迫后期(PEG-6000胁迫75 h)表达量下降;复水后3~6 h上调表达,复水9 h后表达量下调至对照水平。-γECS基因在水分胁迫阶段呈上调表达,复水后表达量下调至对照水平。可见,小麦SAMS、SAMDC和-γECS基因的表达都受水分胁迫诱导,同时,SAMS与SAMDC基因还参与水分胁迫后的复水调节,说明S-腺苷甲硫氨酸代谢途径在小麦抗旱节水中具有重要作用。 |
| 关 键 词： | 小麦 水分胁迫 S-腺苷甲硫氨酸代谢途径 表达模式 S-腺苷甲硫氨酸合成酶(SAMS) S-腺苷甲硫氨酸脱羧酶(SAMDC) γ-谷氨酰半胱氨酸合成酶(-γECS) |
Expression of Genes Related to the S-adenosyl-L-methionine Metabolic Pathways during Water Stress in Wheat |
|
| LI Chang-peng,ZHOU Lin-lin,CHEN Liang,HUANG Lin-zhou,CHEN Xiao-jie,WANG Yu-shen,HU Yin-gang.Expression of Genes Related to the S-adenosyl-L-methionine Metabolic Pathways during Water Stress in Wheat[J].Acta Botanica Boreali-Occidentalia Sinica,2011,31(6):1120-1126. | |
| Authors: | LI Chang-peng ZHOU Lin-lin CHEN Liang HUANG Lin-zhou CHEN Xiao-jie WANG Yu-shen HU Yin-gang |
| Institution: | LI Chang-peng1,ZHOU Lin-lin2,CHEN Liang2,HUANG Lin-zhou2,CHEN Xiao-jie2,WANG Yu-shen2,HU Yin-gang1,2,3,4(1 College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100,China,2 College of Agronomy,3 Yangling Branch of China National Wheat Improvement Centre,4 Shaanxi Key Laboratory of Molecular Biology for Agriculture,China) |
| Abstract: | To study the roles of the S-adenosyl-L-methionine metabolic pathways in drought-tolerance and water use efficiency of wheat,we deployed the semi-quantitative RT-PCR to investigate the expression patterns of the three genes of S-adenosylmethionine synthetase,S-adenosylmethionine decarboxylase and gamma-glutamylcysteine synthetase in the leaves of wheat seedling of Jinmai 47 during PEG-simulated water stress and re-watering after serious water stress.The results showed that the three genes were expressed unde... |
| Keywords: | wheat water stress metabolic pathways of S-adenosyl-L-methionine express profile S-adenosylmethionine synthetase(SAMS) S-adenosylmethionine decarboxylase(SAMDC) gamma-glutamylcysteine synthetase(γ-ECS) |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |