套袋黄冠梨果实花斑病发生与其组织结构变化的关系

以黄冠梨果实为试验材料,在调查套袋处理果实花斑病发生的基础上,研究了套袋及未套袋黄冠梨果实发育过程中的组织结构变化,并分析了发病果实的病部与正常果实组织结构差异。结果显示:(1)套3层袋果实花斑病的发病率为22.5%,而不套袋果实的发生率仅为1.5%。(2)未套袋果实角质层厚,而且呈波纹状分布,并渗入到表皮细胞间隙中,果实发育过程中厚度变化幅度不明显;套袋果实表面的角质层均匀一致,随果实迅速膨大,角质层变薄,且显著薄于未套袋果实,尤其是套3层袋与未套袋果实差异更大;套袋果实表皮细胞呈长方形,且较未套袋果实排列致密;不同套袋处理果实单宁细胞层数随果实发育逐渐减少,在花后90 d后,套袋处理与未套袋处理的厚度无显著差异。(3)套袋果实果肉薄壁细胞排列紧密,胞间隙小,其石细胞大小和密度均较未套袋果实小。(4)套袋果实花斑病发病部位角质层模糊,发病果实正常部位的角质层、表皮层厚度和单宁层均低于正常果实。研究表明,套袋黄冠梨果实表面组织结构发生较明显变化,它对梨果实花斑病的发生有重要影响。

Relationship between Browning Spot and Micro-Structure of Bagged Fruit of Huangguan Pear

On the basis of investigation of browning spot,the micro-structure of Huangguan pear fruit bagged in white one-layer,three-layer and non-bagged fruit was observed,and microstructure difference between pathological site of browning spot fruit and normal fruit was analyzed.The results showed that browning spot incidence on fruit peel of three-layer bagged mature fruit was 22.5 percent,and the rate of non-bagged mature fruit was just 1.5 percent.The cuticle of non-bagged fruit showed thick and corrugated distr...