小麦耐逆基因-TaLEA2转化拟南芥的研究

研究小麦第3组LEA基因中T aLEA2对耐旱和耐盐性能的影响.将小麦第3组LEA基因T aLEA2连接在双元表达载体pB I121 C aM V 35S启动子下游,构建了能在植物中高效表达的载体pB I121-T aLEA2.通过农杆菌介导的真空渗透法,将其转入野生拟南芥中,经抗性筛选及PCR验证,获得T0代转基因植株,并用不同浓度的PEG 4000和N aC l对转基因拟南芥的耐逆性进行检测.结果表明,这些转基因植株可明显改进拟南芥在10%PEG及0.8%N aC l培养基上的生长状态.在实验条件下,转基因拟南芥的耐旱性及耐盐性均有所提高,提示T aLEA2基因在植物水分调节方面有重要作用.

Transformation of a Stress-Tolerant Gene in Wheat,TaLEA2 ,into Arabidopsis thaliana

This study was aimed at studying the drought-and salt-tolerant effects of TaLEA_2 belonging to the third group of LEA genes in wheat.Its method was to combine TaLEA_2 belonging to the third group of LEA genes in wheat onto the downstream of CaMV35S promoter of double-element expression vector (pBI121 and) thus pBI121-TaLEA_2,a vector that was capable of efficiently expressing itself in plants,was constructed.The vector was transformed into Arabidopsis thaliana by Agrobacterium-mediated depressor permeating method and then transgenic Arabidopsis thaliana plants in T_0 were obtained by resistance screening and PCR identification;the stress tolerances of the transgenic Arabidopsis thaliana plants were tested with PEG4000 and NaCl of different concentrations;The test results showed that these transgenic plants presented a remarkably-improved growth on the medium containing 10% PEG4000 and 0.8% NaCl.The conclusion of this study is that under the experimental circumstances,transgenic Arabidopsis thaliana plants get their drought and salt tolerances improved to some extent and TaLEA_2 plays an important role in regulating plant water.