| 青海油菜蜂花粉黄酮类化合物含量的研究 |
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| 引用本文： | 魏永生,郑敏燕,王永宁,石玉平,卢挺.青海油菜蜂花粉黄酮类化合物含量的研究[J].西北植物学报,2004,24(2):301-305. |
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| 作者姓名： | 魏永生 郑敏燕 王永宁 石玉平 卢挺 |
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| 作者单位： | 1. 青海师范大学,化学系,西宁,810008
2. 青海省产品质量监督检验所,西宁,810008 |
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| 基金项目： | 科技部农业科技成果转化资金资助项目(2002630080415)，青海省科技厅资助项目(2002-N-119) |
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| 摘 要： | 采用正交试验设计，研究了青海油菜蜂花粉中黄酮类化合物的最佳提取条件，分别采用HPLC法和比色法测定了黄酮类化合物的含量。最佳提取条件是选用95％的乙醇作为溶剂，以10倍总量的乙醇用量，水浴热回流提取。以槲皮素、山奈酚、异鼠李素为对照品，采用HPLC法，测定出黄酮类化合物平均含量为3．194％；以芦丁为对照物，采用比色法，测定出总黄酮平均含量为3．70％。
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| 关 键 词： | 油菜 蜂花粉 黄酮 HPLC 比色法 |
| 文章编号： | 1000-4025(2004)02-0301-05 |
A study on the content of flavonoids in bee-pollen of Brassica campestris from Qinghai |
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| WEI Yong-sheng,ZHENG Min-yan,WANG Yong-ning,SHI Yu-ping,LU Ting.A study on the content of flavonoids in bee-pollen of Brassica campestris from Qinghai[J].Acta Botanica Boreali-Occidentalia Sinica,2004,24(2):301-305. |
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| Authors: | WEI Yong-sheng ZHENG Min-yan WANG Yong-ning SHI Yu-ping LU Ting |
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| Institution: | WEI Yong-sheng~1,ZHENG Min-yan~1,WANG Yong-ning~1,SHI Yu-ping~1,LU Ting~2 |
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| Abstract: | The optimum condition of extracting the flavonoids in bee-pollen of Brassica campestris Qinghai was studied by the orthogonal experiment design. The content of flavonoids in bee-pollen was determined by HPLC and colorimetry. The optimum conditions were as follows: the addition of solvent (95% ethanol) was ten times the addition of solute, then reflux in heated water-bath. The average content of flavonoids was 3.194% by HPLC, 3.70% by colorimetry with quercetin, kaempferol , isorhamnetin and rutin as standard samples. |
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| Keywords: | Brassica campestris bee-pollen flavonoids HPLC colorimetry |
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