锌锰钛对接种放线菌剂甜瓜幼苗生长及诱导抗病性的影响

以陕甜3号甜瓜幼苗为材料,采用基质育苗、大田栽培法,研究了2种放线菌剂(Act28和康照)育苗接种和育苗移栽双重接种条件下,微量元素锌、锰、钛对甜瓜植株生长及诱导抗病性的影响.结果显示:(1)与单施菌剂相比,在育苗接种Act28并配施微量元素条件下甜瓜植株总鲜重、根鲜重和蔓叶鲜重分别显著增加31.5%、39.5%和31.5%,在双重接种Act28配施微量元素条件下分别显著增加40.5%、17.6%和40.7%.(2)与单纯菌剂接种相比,在育苗接种和双重接种Act28并配施微量元素下甜瓜叶片过氧化物酶活性分别降低4.4%和10.2%,丙二醛含量分别增加10.9%和16.6%;甜瓜叶片多酚氧化酶、过氧化物酶活性及可溶性蛋白含量在育苗接种康照并配施微量元素下分别较育苗接种康照降低2.2%、38.0%和5.0%,丙二醛含量增加17.7%.(3)甜瓜根系多酚氧化酶活性和丙二醛含量在育苗接种Act28配施微量元素处理下较育苗接种分别显著增加30.7%和42.6%,而在Act28双重接种配施微量元素与双重接种处理间无明显变化;甜瓜根系过氧化物酶活性在育苗接种Act28、康照与微量元素配施处理下,分别较2种菌剂育苗接种处理显著降低14.7%、34.3%,苯丙氨酸解氨酶活性分别明显降低14.9%、21.9%.研究表明,菌剂Act28育苗接种或育苗移栽双重接种条件下,锌、锰、钛溶液对甜瓜植株生长具有显著的促进作用,且微量元素效应与接入的菌剂种类及接种方式有关;微量元素溶液对甜瓜防卫酶活性及抗性物质含量等无显著影响或具有一定程度的负效应,从而导致甜瓜诱导抗病性下降.

Effects of Microelements Admixture of Zine,Manganese and Titanium on the Growth and Induced Disease Resistance of Cucumis melo L. under Condition of Actinomycete Inoculation

A field experiment was conducted in order to assess the growth-promoting and induced disease-resistant effect of the microelements admixture of zine,manganese and titanium on ’Shaantian NO.3’ Cucumis melo L.under condition of the inoculation of 2 biocontrol actinomycete agents.The results showed that:(1)When microelements admixture was applied with agent Act28 culture inoculation and twice inoculation,the fresh weight of total plant,root and aerial parts of C.melo L.were increased by 31.5% and 40.5%,39.5% and 17.6%,31.5% and 40.7%,respectively,compared with single biocontrol agents inoculation;(2)The POD activity of melon leaves decreased by 4.4% and 10.2%,while the MDA content increased by 10.9% and 16.6%,when microelements admixture was applied with agent Act28 culture inoculation and twice inoculation treatments,compared to the inoculation with biocontrol agent Act28 only;With Kangzhao culture and microelements admixture addition,PPO,POD activities and soluble protein content decreased by 2.2%,38.0% and 5.0%,respectively,and MDA content increased by 17.7%,compared to Kangzhao culture inoculation treatments.(3)Compared with the culture inoculation of agent Act28 only,PPO activity of melon roots significantly increased by 30.7% and the membrane lipid peroxidation aggravated with the MDA content obviously increased by 42.6% in the treatment of microelements admixture applied with agent Act28 culture inoculation,while no significant difference were found in the twice culture inoculation of Act28;In the microelements admixture applied groups,the POD activity of melon roots obviously decreased by 14.7% and 34.3%,respectively,compared with the culture inoculation of agents Act28,Kangzhao treatments.The PAL activity also significantly decreased by 14.9% and 21.9%,respectively,with the same treatments.The results indicated that when microelements admixture was applied with agent Act28 culture inoculation and twice inoculation,the texted microelements admixture showed obvious growth promoting effect on C.melo L.;The microelements admixture efficiency were related to the agents and inoculation ways,the activities of defense enzymes of C.melo L.showed little distinct variety even negative effect after application of microelements admixture with the inoculation of biocontrol agents,which would lead to reduce disease resistant ability of C.melo L..