草莓高频离体再生体系的研究

以6个草莓品种为试材,研究了影响草莓不定芽再生的各种因素,建立离体叶片高效再生系统。结果表明,外植体基因型、激素种类及配比、叶龄等是影响草莓再生的主要因子,其中‘鬼露甘’叶片最佳芽诱导培养基为MS 2.0 mg/L 6-BA 0.1 mg/L IBA,‘嫜姬’叶片愈伤组织的诱导以MS 3 mg/L 6-BA 0.2 mg/L 2,4-D较好,而且1周左右的暗培养可以防止外植体的褐化。芽伸长的最适培养基为MS 0.5 mg/L 6-BA 0.5 mg/L IBA,生根的最适培养基为MS 0.2 mg/L IBA,试管苗移栽后成活率为87%。

in vitro Efficient Regeneration System of Strawberry

Six cultivars of strawberry(Fengxiang,Guilugan,Quanmingxiang,Zhangji,Hali,Xingxiang)were investigated for factors affecting the regeneration of adventitious bud.The results indicated that the genotype,hormones and seedling ages were the main factors affecting regeneration.The optimum shoot inducing medium of the leaves from Guilugan was MS 2.0 mg/L 6-BA 0.1 mg/L IBA,the better medium for the induction of callus from the leaves of Zhangji was MS mg/L 6-BA3 0.2 mg/L 2,4-D;the optimum medium for shoot elongation was MS 0.5 mg/L 6-BA 0.5 mg/L IBA,the optimum root inducing medium were MS 0.2 mg/L IBA.The survival rate of the plantlets transplanted was 87%.Dark incubation for 1 week can reduce the browning of cultures.A highly efficient in vitro regeneration system from leaf explants was established.