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干旱胁迫下沙生灌木花棒的抗氧化保护响应研究
引用本文:韩刚,党青,赵忠.干旱胁迫下沙生灌木花棒的抗氧化保护响应研究[J].西北植物学报,2008,28(5):1007-1013.
作者姓名:韩刚  党青  赵忠
作者单位:西北农林科技大学,林学院,陕西杨陵,712100
摘    要:采用盆栽试验,测定了在不同土壤水分胁迫程度和胁迫时间下花棒苗抗氧化保护酶(SOD、CAT、POD、APX)活性和抗氧化物(AsA、GSH、Car)含量,并用隶属函数值法综合评价了试验条件下花棒苗的抗氧化能力。结果显示:(1)与同期适宜水分处理相比,花棒苗的抗氧化保护酶活性在中度和重度干旱胁迫30d时均显著增加;胁迫60d时,POD活性均显著降低,而其余酶活性仍增加(重度胁迫)或显著增加(中度胁迫);胁迫90d时,中度胁迫处理除APX活性持平外,其余酶活性仍显著升高,而重度胁迫处理的SOD活性持平、POD活性显著增加、CAT和APX活性均显著降低。(2)相对于同期适宜水分处理,干旱胁迫30d时,中度胁迫处理的花棒苗AsA、Car、GSH含量分别显著增加、稍有增加和显著降低,而重度胁迫的AsA显著增加,Car和GSH含量均显著降低;胁迫60d时,中度胁迫处理的AsA、Car、GSH含量分别显著增加、稍有降低和稍有提高,而重度胁迫的AsA、Car、GSH含量分别显著增加、稍有增加和显著降低;胁迫90d时,中度胁迫处理的AsA含量显著增加,Car和GSH含量均稍有提高,而重度胁迫的AsA含量稍有下降,Car和GSH含量则持平。(3)花棒苗的MDA含量在干旱胁迫过程中均比适宜水分处理增加,且中后期达到显著水平。(4)花棒苗抗氧化能力在干旱胁迫30d表现为重度干旱>中度干旱>适宜水分,胁迫60d为中度干旱>重度干旱>适宜水分,胁迫90d时为中度干旱>适宜水分>重度干旱。研究表明,花棒苗在中度干旱胁迫下能调动自身抗氧化保护酶类与抗氧化物质并提高其抗氧化能力,消除或减轻干旱诱导的活性氧毒害;重度干旱胁迫下的花棒苗在胁迫前中期亦能增强自身的抗氧化能力,但在胁迫后期因部分活性氧清除机制受到破坏而使抗氧化能力降低。

关 键 词:干旱胁迫  花棒  抗氧化保护

Response of Antioxidation Protection System of Hedysarum scoparium to Drought Stress
HAN Gang,DANG Qing,ZHAO Zhong.Response of Antioxidation Protection System of Hedysarum scoparium to Drought Stress[J].Acta Botanica Boreali-Occidentalia Sinica,2008,28(5):1007-1013.
Authors:HAN Gang  DANG Qing  ZHAO Zhong
Abstract:By using potted-seedlings some kinds of enzyme (SOD,CAT,POD,APX) and metabolites (AsA,GSH,Car) involved with antioxidation protection system of Hedysarum scoparium Fisch.et Mey.seedlings were measured at the different time and under different soil moisture treatments.Applying subordinate function values method the antioxidant capability of H.scoparium is evaluated comprehensively under experimental condition.The results showed:(1) compared with suitable soil moisture treatment,the activities of all tested antioxidant enzymes are increased significantly on the 30th day under medium and severe drought treatments.On the 60th day POD activity is decreased but the other enzymes are still increased (under severe drought treatment) or increased significantly (under medium drought treatment).On the 90th day under medium drought treatment the activities of all tested enzymes are still increased significantly excpet that APX activity keeps on suitable soil moisture level and under severe drought treatment SOD activity keeps on suitable soil moisture level,POD activity is increased significantly,CAT and APX activities are decreased significantly.(2)Compared with suitable soil moisture treatment,AsA,Car and GSH contents are respectively increased significantly,increased little and decreased apparently on the 30th day under medium drought treatment but AsA content is increased significantly and Car and GSH contents are decreased significantly under severe drought treatment.On the 60th day AsA,Car and GSH contents are significantly increased respectively,decreased little and increased little but increased significantly,increased little and decreased significantly under severe drought treatment.On the 90th day AsA content is increased significantly and Car and GSH contents keeps on suitable soil moisture level under severe drought treatment.(3)Compared with suitable soil moisture treatment,MDA content is always increased under drought treatment and reach significant level at medium and last period.(4)On the 30th day of soil moisture treatment the antioxidant capability of H.scoparium is under severe drought>medium drought>suitable soil moisture.On the 60th day,it is under medium drought>severe drought>suitable soil moisture,and on the 90th day,it is under medium drought>suitable soil moisture>severe drought.The research finds under medium drought treatment the antioxidant enzyme and metabolites of H.scoparium is adjusted to increase its antioxidant capability in 90 days.This can avoid active oxygen species injuring.Under severe drought treatment H.scoparium responds to oxidation stress to increase its antioxidant capability considerably in initial 60 days but the capability has already fall down obviously on the 90th day because some active oxygen scavenging have been destroyed.
Keywords:drought stress  Hedysarum scoparium Fisch  et Mey    antioxidation protection
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