|
|||||
|
|
| 应用基因芯片检测酸味抗病甜瓜果实基因表达 | |
| 引用本文： | 张红,张志斌,王怀松,贺超兴,张显,吴明珠,伊鸿平.应用基因芯片检测酸味抗病甜瓜果实基因表达[J].西北植物学报,2009,29(4). |
| 作者姓名： | 张红 张志斌 王怀松 贺超兴 张显 吴明珠 伊鸿平 |
| 作者单位： | 1. 西北农林科技大学,园艺学院,陕西杨陵,712100;中国农业科学院蔬菜花卉研究所,北京,100081 2. 中国农业科学院蔬菜花卉研究所,北京,100081 3. 西北农林科技大学,园艺学院,陕西杨陵,712100 4. 新疆农业科学院哈密瓜研究中心,乌鲁木齐,830000 |
| 摘 要： | 利用Melon cDNA array ver1.0检测新疆厚皮甜瓜(Cucumis melo var.ameri)果实基因表达的可行性,并检测了经60Coγ射线辐射诱变后的新疆厚皮甜瓜酸味抗病变异株成熟果实基因的表达.结果显示:该芯片平均能够检测新疆厚皮甜瓜基因2 008个,检测出的基因占该芯片基因探针总数的65.4%;检测酸味抗病变异株上调表达的基因251个,占检出基因总数的12.5%;下调表达的基因224个,占检出基因总数的11.16%.利用RT-PCR验证芯片结果的可靠性,结果表明,用Melon cDNA array ver 1.0检测新疆厚皮甜瓜成熟果实基因表达水平是可行的. |
| 关 键 词： | 甜瓜 基因芯片 实时荧光定量PCR 果实基因表达 |
Identification of Fruit Genes Expression in Sourness Taste and Resistance Melon by Microarray Analysis |
|
| ZHANG Hong,ZHANG Zhi-bin,WANG Huai-song,HE Chao-xing,ZHANG Xian,WU Ming-zhu,YI Hong-ping.Identification of Fruit Genes Expression in Sourness Taste and Resistance Melon by Microarray Analysis[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(4). | |
| Authors: | ZHANG Hong ZHANG Zhi-bin WANG Huai-song HE Chao-xing ZHANG Xian WU Ming-zhu YI Hong-ping |
| Institution: | 1 College of Horticulture;Northwest A&F University;Yangling;Shaanxi 712100;China;2 Institute of Vegetable and Flowers;Chinese Academy of Agricultural Sciences;Beijing 100081;3 Melon Research Centre;Xinjiang Academy of Agricultural Sciences;Urumqi 830000;China |
| Abstract: | The feasibility of using Melon cDNA array ver1.0 to detect the gene expression in mature fruit of Cucumis melo var.ameri with sourness taste and resistance variation.The results showed that the melon cDNA array ver1.0 could detect the expression of 2008 genes in Cucumis melo var.ameri variety of sourness taste and resistance.The detected genes accounted for 65.4% of the total 3068 probe groups.251 up-regulated and 224 down-regulated genes were detected in variety of sourness taste and resistance,accounting ... |
| Keywords: | Cucumis melo microarray RT-PCR fruit gene expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《西北植物学报》浏览原始摘要信息 | |
| 点击此处可从《西北植物学报》下载免费的PDF全文 | |