UV-B对不同发育时期离体蓝莓主要果实品质及相关酶活性的影响

该研究以幼果期、白果期、转色期的离体‘北陆’蓝莓果实为试材,设置0(CK)、5、10、15min紫外光辐照处理,24h后取样分析蓝莓果实中可溶性糖、总酚、类黄酮和花青苷含量,以及苯丙氨酸裂解酶(PAL)和查尔酮异构酶(CHI)活性的变化,探究UV-B紫外照射处理对不同发育时期蓝莓主要果实品质及相关酶活的影响。结果显示:(1)对于幼果期蓝莓,5min UV-B处理可显著增加果实内可溶性糖含量;10min UV-B处理果实PAL活性增加效果最为显著;15min UV-B处理对果实总酚和花青苷积累的促进作用最大,但显著降低了类黄酮含量和CHI活性。(2)对于白果期蓝莓,5min UV-B处理显著增加了果实类黄酮含量和CHI活性,10min处理使果实可溶性糖和总酚含量较对照分别增加25%和18%;15min处理对果实花青苷含量和PAL活性影响作用最大。(3)对于转色期蓝莓,各处理除果实可溶性糖及类黄酮含量降低外,其余物质含量均显著增加。(4)UV-B处理并未改变果实发育过程中可溶性糖、总酚、类黄酮和花青苷含量及PAL、CHI酶活性的积累规律。(5)蓝莓果内PAL活性与其可溶性糖、总酚和类黄酮的积累呈极显著正相关关系,而CHI活性仅与其可溶性糖呈极显著正相关。研究表明,UV-B辐照处理促进了幼果期和白果期可溶性糖的积累,也能促进不同发育时期蓝莓果实总酚和花青苷及白果期类黄酮的积累,对蓝莓果实主要品质能够产生积极的影响。

Effects of UV-B Treatment on the Major Quality of Blueberry and Related Enzyme Activities in Different Developmental Stages

To explore the UV-B ultraviolet irradiation treatment on the major fruit quality and related enzyme activities in different developmental stages of blueberry,we chose Northland as plant material.The radiation time was 5 min,10 min and 15 min,respectively,and taking no irradiation as control.The result shows that:(1)with UV-B treated young fruit of blueberries,the soluble sugar content significantly increased 47% compared with control by 5 min treatment.The PAL activity increased the most significantly by 10 min treatment.The total phenols and anthocyanin accumulation increased 36% and 31% comparing with the control by 15 min treatment,but flavonoid content and CHI activity reduced significantly.(2)With UV-B treatment ginkgo blueberry,the flavonoids and CHI activity increased significantly by 5 min treatment.The soluble sugar and total phenolic contents increased 25% and 18% than the control by 10 min treatment.The anthocyanin and PAL activity increased the most significantly by 15 min treatment reaching at 48% and 151% comparing with the control.(3)With UV-B treated verarison blueberries 10 min,the soluble sugar and flavonoid contents decreased and the other material content increased significantly.(4)UV-B treatment did not change the regularization during the fruit development of the contents of soluble sugar,total phenols,flavonoids,anthocyanins,and PAL,CHI.(5)The activity of PAL was positively correlated to soluble sugar,total phenolic and flavonoid accumulation.But the activity of CHI was only positively correlated to soluble sugar.Research shows UV-B treatment made soluble sugar content increase at low dose and decrease at high dose.UV-B irradiation could promote the accumulation of total phenols,anthocyanin and flavonoids during ginkgo blueberries,decrease the CHI activity in young fruit,increase the PAL and CHI activities in ginkgo and verarison fruit,also could make positive influence about the major quality of blueberry.