菊花不同生长阶段不同器官POD和EST同工酶比较

采用过氧化物酶(POD)、酯酶(EST)2个酶系统的12个同工酶位点,分析了4个菊花品种营养生长和生殖生长阶段不同器官(嫩叶、老叶、嫩茎、木质化茎)的同工酶变化.结果表明:(1)4个品种共有16种POD酶带,15种EST酶带;(2)菊花的POD和EST具有组织特异性和阶段特异性,其中以嫩叶的酶带最多,其次为老叶,再次为嫩茎,而木质化茎的酶带最少;(3)与生殖生长阶段相比,营养生长阶段的POD酶带更清晰,更整齐,分离更好,但生殖生长阶段的EST同工酶比营养生长阶段的更清晰;(4)营养生长阶段的嫩叶最适合用于菊花POD同工酶分析,而EST同工酶研究宜取生殖生长时期的嫩叶.

Isozyme Analysis of Stem and Leaf in Chrysanthemum during Vegetative and Reproductive Growth Stages

Twelve peroxidase isozyme(POD) and esterase isozyme(EST) loci were obtained from different organs of 4 chrysanthemum cultivars during vegetative and reproductive growth stages.The result showed that:(1)16 POD bands and 15 EST bands were respectively obtained;(2)POD and EST were tissue specific and process specific.Among young leaf,old leaf,young stem and xyloid stem,the most number of isozyme band were observed in young leaf,and the least number of isozyme band were in xyloid stem;(3)There were more peroxidase isozyme bands obtained during vegetative stage than that during reproductive stage,and esterase isozyme bands during reproductive stage were more clean than that during vegetative stage;(4)The young leaf during vegetative growth stage is best for POD research,while the one during reproductive growth stage is the best for EST research.