|
|||||
|
|
| 狗蔷薇茎尖再生体系的优化 | |
| 引用本文： | 余晓丽,康洁,李玉英,杨玉珍.狗蔷薇茎尖再生体系的优化[J].西北植物学报,2005,25(8):1536-1539. |
| 作者姓名： | 余晓丽 康洁 李玉英 杨玉珍 |
| 作者单位： | 1. 南阳师范学院,生物系,河南南阳,473061 2. 商丘师范学院,生物系,河南商丘 476000 3. 河南南阳农校,园艺系,河南南阳,473000 |
| 基金项目： | 南阳师范学院2003年应用与科技开发项目（nytc200318）;河南省南阳市2004年科技攻关项目（200404016） |
| 摘 要： | 采用正交设计及方差分析对影响狗蔷薇植株再生体系的因素进行了优化研究,结果表明:影响狗蔷薇植株再生的最主要因素是NAA和BAP,其次为AgNO3和蔗糖.适宜狗蔷薇茎尖再生的培养基为:MS+BAP1.5mg.L-1+NAA0.1mg.L-1+AgNO33.5mg.L-1+蔗糖40g.L-1+琼脂6.0g.L-1,植株再生频率高达96.3%,增殖系数为6.5. |
| 关 键 词： | 狗蔷薇 茎尖 再生体系 正交设计 方差分析 |
| 文章编号： | 1000-4025（2005）08-1536-04 |
| 收稿时间： | 03 25 2005 12:00AM |
| 修稿时间： | 2005-03-25 |
Optimization of the Regeneration Protocol of Rosa canina cv.‘Edelcaninas’Meristem |
|
| YU Xiao-li,KANG Jie,LI Yu-ying,YANG Yu-zhen.Optimization of the Regeneration Protocol of Rosa canina cv.‘Edelcaninas’Meristem[J].Acta Botanica Boreali-Occidentalia Sinica,2005,25(8):1536-1539. | |
| Authors: | YU Xiao-li KANG Jie LI Yu-ying YANG Yu-zhen |
| Institution: | YU Xiao-li~1,KANG Jie~2,LI Yu-ying~1,YANG Yu-zhen~3 |
| Abstract: | Orthogonal design and variance analysis were used to study the optimization of the factors for the regeneration protocol of Rosa canina cv. 'Edelcaninas' and the results showed that the major factors affecting the regeneration of Rosa canina plants were NAA and BAP in the first place and AgNO_3, and sucrose in the next place. The medium suitable for the regeneration of Rosa canina meristem was MS+1.5 mgL~(-1) BAP+0.1 mgL~(-1)NAA+ 3.5 mgL~(-1) AgNO_3 + 40 gL~(-1) sucrose+6.0 gL~(-1) agar with its regeneration rate amounting to as high as 96.3% and its propagation rates reaching 6.5. |
| Keywords: | Rosa canina meristem regeneration system orthogonal design variance analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《西北植物学报》浏览原始摘要信息 | |
| 点击此处可从《西北植物学报》下载免费的PDF全文 | |